Fig. S3
Pronephros repair is independent of EMT.
In situ hybridization for genes characteristic for epithelial-to-mesenchymal transition (EMT) or de-differentiation. Two-day-old embryos were injured, fixed 4 hours after wounding, and stained for EMT-typical genes (N-myc, vimentin, snail, netrin and mmp9), or for genes characteristic for non-differentiated pronephric cells (pax2.1 and tbx2b). None of the markers showed up-regulation in the repair region, outlined by a black bracket. The staining for mmp9 and snail was performed in combination with staining for cdh17 labeling the pronephric cells in red (Scale bar, 100 μm).