ZFIN ID: ZDB-FIG-181003-18
Yakulov et al., 2018 - CXCL12 and MYC control energy metabolism to support adaptive responses after kidney injury. Nature communications   9:3660 Full text @ Nat. Commun.
Anatomical Terms:
Stage: Long-pec
Observed In:
Stage: Long-pec

Fig. 5

Cxcr4b expression in response to injury. a The GFP-RFP-tagged Cxcr4b (cxcr4b:cxcr4b-tFT) was up-regulated in cells adjacent to the laser-induced injury. The arrowhead points to the corpuscle of Stannius. Arrows point to RFP-positive cells, accumulating distally after the injury. Sequential frames were taken from a time-lapse video at t1 = 96 min, t2 = 120 min, and t3 = 160 min after injury (scale bar, 20 µm). b In situ hybridization revealed up-regulated cxcr4b expression in the leading ends of the regenerating tubule 2 h after laser-induced ablation. The cxcr4b up-regulation is outlined in a dashed box. Note that cxcr4b is strongly expressed in the corpuscle of Stannius (arrow) and in the lateral line. c Magnification of the injury region depicted in b. The white arrows point towards the up-regulated cxcr4b, the red arrowhead towards the corpuscle of Stannius

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
cxcr4b WT light ablation: pronephros Long-pec corpuscles of Stannius ISH
Long-pec lateral line ISH
Long-pec pronephros ISH
EGFP zf512Tg ; uf1Tg light ablation: pronephros Long-pec pronephros IFL
RFP zf512Tg ; uf1Tg light ablation: pronephros Long-pec corpuscles of Stannius IFL
Long-pec pronephros IFL
Long-pec pronephros regenerating tissue IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT light ablation: pronephros Long-pec pronephros cxcr4b expression increased amount, abnormal
zf512Tg ; uf1Tg light ablation: pronephros Long-pec pronephros RFP expression increased amount, abnormal
ZFIN wishes to thank the journal Nature communications for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Nat. Commun.