ZFIN ID: ZDB-FIG-181003-18
Yakulov et al., 2018 - CXCL12 and MYC control energy metabolism to support adaptive responses after kidney injury. Nature communications   9:3660 Full text @ Nat. Commun.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage: Long-pec
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Long-pec

Fig. 5

Cxcr4b expression in response to injury. a The GFP-RFP-tagged Cxcr4b (cxcr4b:cxcr4b-tFT) was up-regulated in cells adjacent to the laser-induced injury. The arrowhead points to the corpuscle of Stannius. Arrows point to RFP-positive cells, accumulating distally after the injury. Sequential frames were taken from a time-lapse video at t1 = 96 min, t2 = 120 min, and t3 = 160 min after injury (scale bar, 20 µm). b In situ hybridization revealed up-regulated cxcr4b expression in the leading ends of the regenerating tubule 2 h after laser-induced ablation. The cxcr4b up-regulation is outlined in a dashed box. Note that cxcr4b is strongly expressed in the corpuscle of Stannius (arrow) and in the lateral line. c Magnification of the injury region depicted in b. The white arrows point towards the up-regulated cxcr4b, the red arrowhead towards the corpuscle of Stannius

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
cxcr4b WT light ablation: pronephros Long-pec corpuscles of Stannius ISH
Long-pec lateral line ISH
Long-pec pronephros ISH
EGFP zf512Tg ; uf1Tg light ablation: pronephros Long-pec pronephros IFL
RFP zf512Tg ; uf1Tg light ablation: pronephros Long-pec corpuscles of Stannius IFL
Long-pec pronephros IFL
Long-pec pronephros regenerating tissue IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT light ablation: pronephros Long-pec pronephros cxcr4b expression increased amount, abnormal
zf512Tg ; uf1Tg light ablation: pronephros Long-pec pronephros RFP expression increased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Nature communications for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Nat. Commun.