FIGURE

Fig. 3

ID

ZDB-FIG-180905-21

Publication

Genthe et al., 2017 - R-spondin-1 is required for specification of hematopoietic stem cells through Wnt16 and Vegfa signaling pathways

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Fig. 3

Notch pathway gene expression in Rspo1 morphants. Wild-type (A,D,G,J,M,P) or Rspo1 MO-injected animals (B,E,H,K,N,Q) were examined by WISH for expression of the Notch ligands dlc (A,B), dld (D,E), or Notch receptors notch1a (G,H), notch1b (J,K), notch2 (M,N) and notch3 (P,Q) at the time points indicated. Compared with wild-type (A,D), dlc and dld are specifically decreased (B,E) in the formed somites (below red bars), but not the pre-somitic mesoderm. Notch receptor expression is unaffected. Flat mount dorsal views of the trunk or lateral views at 160× magnification. Numbers of embryos displaying the depicted phenotype are indicated. (C,F,I,L,O,R) Histograms illustrate percentages of phenotypes. (S,T) Decreased dlc and dld mRNA in Rspo1 knockdown (Rspo1 MO) was confirmed by qRT-PCR, normalized to expression of ef1a, of transcript isolated from dissected embryo trunks (mean±s.e.m.). *P≤0.0006, determined by one-way ANOVA.

Expression Data

Genes:	dlc dld notch1a notch1b notch2 notch3
Fish:	WT WT + MO3-rspo1
Knockdown Reagent:	MO3-rspo1
Anatomical Terms:	segmental plate somite whole organism
Stage Range:	14-19 somites to Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO3-rspo1
Knockdown Reagent:	MO3-rspo1
Observed In:	somite whole organism
Stage:	14-19 somites

Phenotype Detail

Acknowledgments

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