FIGURE

Fig. 5

ID

ZDB-FIG-180905-23

Publication

Genthe et al., 2017 - R-spondin-1 is required for specification of hematopoietic stem cells through Wnt16 and Vegfa signaling pathways

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Fig. 5

Dlc, dld and vegfaa-165 can rescue HSC specification in Rspo1 morphants. Expression of the HSC markers runx1 (A,D,G,J,M,P,S) and cmyb (B,E,H,K,N,Q,T), and the T lymphocyte marker rag1 (C,F,I,L,O,R,U) in the thymus were examined at 24 hpf, 36 hpf, and 96 hpf as indicated, by WISH in wild-type embryos (A-C), or embryos injected with 5 ng Rspo1 MO (D-F), or 5 ng Rspo1 MO and mRNAs encoding candidate rescue genes dlc (100 ng; G-I), dld (50 ng; J-L), dlc+dld (50 ng and 25 ng, respectively; M-O), vegfaa-165 (100 ng; P-R) or vegfaa-121 (100 ng; S-U). dlc and dld mRNA were independently able to partially rescue HSCs. Co-injection of dlc and dld caused a more complete rescue of HSCs. vegfaa-165, but not vegfaa-121, was able to rescue HSCs. Red arrowheads indicate runx1⁺ (A,D,G,J,M,P,S), and cmyb⁺ (B,E,H,K,N,Q,T) HSCs, or rag1⁺ T lymphocytes (C,I,L,O,R), when visible. Numbers of embryos displaying the depicted phenotype are indicated. Lateral views of the mid trunk at 160× magnification.

Expression Data

Genes:	myb rag1 runx1
Fish:	WT WT + MO3-rspo1
Knockdown Reagent:	MO3-rspo1
Anatomical Terms:	dorsal aorta hematopoietic stem cell thymus T cell
Stage Range:	Prim-5 to Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO3-rspo1
Knockdown Reagent:	MO3-rspo1
Observed In:	dorsal aorta hematopoietic stem cell thymus T cell
Stage Range:	Prim-5 to Day 4

Phenotype Detail

Acknowledgments

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