FIGURE

Fig. 2

ID

ZDB-FIG-180905-20

Publication

Genthe et al., 2017 - R-spondin-1 is required for specification of hematopoietic stem cells through Wnt16 and Vegfa signaling pathways

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Fig. 2

Rspo1 is required for normal wnt16 expression. Wild-type (WT; A,D,G) or Rspo1 MO-injected animals (B,E,H) were examined by WISH for expression of wnt16 at the time points indicated. Wnt16 is natively found in the dorsal anterior domain of each formed somite, overlapping the rspo1 expression domain, and is diminished in Rspo1 MO-injected animals. Black arrows indicate areas of lost wnt16 expression. Numbers of embryos displaying the depicted phenotype are indicated. (C,F,I) Histograms illustrate percentages of phenotypes. Lateral views of the mid trunk at 160× magnification. (J) qRT-PCR of wnt16 transcript at 18 hpf, 20 hpf, and 24 hpf, normalized to expression of ef1a, reveals decreased wnt16 in Rspo1 knockdown (Rspo1 MO) compared with wild-type (WT) (mean±s.e.m.). *P≤0.0189, determined by one-way ANOVA.

Expression Data

Gene:	wnt16
Fish:	WT WT + MO3-rspo1
Knockdown Reagent:	MO3-rspo1
Anatomical Terms:	somite whole organism
Stage Range:	14-19 somites to Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO3-rspo1
Knockdown Reagent:	MO3-rspo1
Observed In:	somite whole organism
Stage Range:	14-19 somites to Prim-5

Phenotype Detail

Acknowledgments

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