FIGURE

Fig. S5

ID

ZDB-FIG-180823-54

Publication

Ghosh et al., 2018 - Analysis of novel caudal hindbrain genes reveals different regulatory logic for gene expression in rhombomere 4 versus 5/6 in embryonic zebrafish

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Fig. S5

SU5402 disrupts embryogenesis in hoxb1b mutants. Wildtype (i) and hoxb1b mutant (ii) zebrafish embryos were treated with SU5402 and assayed at various developmental stages by brightfield microscopy (A, B, F, H), or ISH to detect expression of efnb2a/krox20 (C), meis1a/krox20 (D), irx7/krox20 (E) or greb1l/krox20 (G). Note that defects in development are readily detectable in hoxb1b mutants treated with 50uM SU5402 (Aii, Bii), but not in WT embryos treated with SU5402 (Ai, Bi), nor in hoxb1b mutants treated with DEAB (Biii). As a result of these severe developmental defects, hoxb1b mutant embryos treated with SU5402 showed no specific staining for the r4 genes tested. (PDF 853 kb)

Expression Data

Genes:	efnb2a egr2b greb1l irx7 meis1a
Fish:	WT hoxb1b^um197/um197
Condition:	chemical treatment by environment: SU5402
Anatomical Terms:	rhombomere 3 rhombomere 4 rhombomere 5
Stage:	10-13 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT hoxb1b^um197/um197
Condition:	chemical treatment by environment: SU5402
Observed In:	multicellular organism development rhombomere 3 rhombomere 4 rhombomere 5 whole organism
Stage Range:	90%-epiboly to 10-13 somites

Phenotype Detail

Acknowledgments

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