FIGURE

Fig. 7

ID

ZDB-FIG-180823-50

Publication

Ghosh et al., 2018 - Analysis of novel caudal hindbrain genes reveals different regulatory logic for gene expression in rhombomere 4 versus 5/6 in embryonic zebrafish

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Fig. 7

gas6, gbx1, sall4, egfl6, celf2 and greb1l function is not required for r4-r6 formation. ISH for hindbrain markers (i) hoxb1a (blue, r4) and krox20 (red r3/5), (ii) pax2 (MHB), krox20 (r3/5) and hoxd4a (r7-anterior spinal cord), and immunostaining for neuronal markers detecting (iii) abducens motor neurons (four green dots in white boxes) in r5/r6 and (iv) Mauthner neurons (white arrows) in r4 was carried out on embryos collected from an a cross of WT fish, b cross of hoxb1a heterozygous mutants, c cross of gas6 homozygous mutants, d cross of gbx1 heterozygous mutants, e cross of greb1l homozygous mutants, f cross of a celf2 heterozygous and a homozygous mutant, g cross of egfl6 heterozygous mutants and h cross of sall4 homozygous mutants. All embryos are oriented in dorsal view with anterior to the top. Embryos collected at 14hpf and 18hpf were imaged as whole-mounts. 48hpf embryos were flat-mounted for imaging

Expression Data

Genes:	egr2b hoxb1a hoxd4a pax2a
Fish:	WT celf2^{sa33469/sa33469} egfl6^{sa21615/sa21615} gas6^um296/um296 gbx1^um300/um300 greb1l^{sa17608/sa17608} hoxb1a^um191/um191 sall4^{sa14110/sa14110}
Anatomical Terms:	midbrain hindbrain boundary rhombomere 3 rhombomere 4 rhombomere 5 rhombomere 7 spinal cord neural tube
Stage Range:	10-13 somites to 14-19 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	hoxb1a^um191/um191
Observed In:	rhombomere 4 rhombomere 4 Mauthner neuron
Stage:	10-13 somites

Phenotype Detail

Acknowledgments

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