FIGURE

Fig. 3

ID

ZDB-FIG-180713-10

Publication

Zhang et al., 2018 - The DEAD box RNA helicase Ddx39ab is essential for myocyte and lens development in zebrafish

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Fig. 3

Loss of ddx39ab results in defective skeletal muscle differentiation in zebrafish. (A) Immunostaining demonstrates the organization of myofilaments in wild-type and ddx39ab mutant embryos at 24 hpf. Anti-MHC antibody (MF20) labels thick (myosin) filaments and F-actin was visualized by phalloidin staining. Scale bars: 20 μm. (B) RNA in situ hybridization for myogenic regulatory gene expression in wild-type and ddx39ab mutant zebrafish embryos at 32 hpf. (C,D) RNA in situ hybridization and qPCR analysis for myocyte structural gene expression in wild-type and ddx39ab mutant zebrafish embryos at 32 hpf. (B,C) Lateral views with anterior to the left. (D) Transverse section with dorsal side to top. At least 15 (A) or 20 (B-D) embryos of each genotype were analyzed and representative samples are shown. For qPCR results, data are mean±s.e.m. n.s, not significant. **P<0.01, ***P<0.001.

Expression Data

Genes:	casq1a myf5 myhz2 myod1 myog slc25a4 smyhc1 tnnt2d
Antibody:	Ab-MF20
Fish:	WT ddx39ab^{nju203Gt/nju203Gt}
Anatomical Terms:	muscle myosin filament somite whole organism
Stage Range:	Prim-5 to Prim-15

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ddx39ab^{nju203Gt/nju203Gt}
Observed In:	muscle myofilament somite whole organism
Stage Range:	Prim-5 to Prim-15

Phenotype Detail

Acknowledgments

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