ZFIN ID: ZDB-FIG-180627-50
Mukaigasa et al., 2018 - Nrf2 activation attenuates genetic endoplasmic reticulum stress induced by a mutation in the phosphomannomutase 2 gene in zebrafish. Proceedings of the National Academy of Sciences of the United States of America   115(11):2758-2763 Full text @ Proc. Natl. Acad. Sci. USA
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Conditions:
Knockdown Reagents:
Anatomical Terms:
Stage: Day 5
PHENOTYPE:
Fish:
Conditions:
Knockdown Reagents:
Observed In:
Stage: Day 5

Fig. 3

PERK-dependent activation of Nrf2 in 5-dpf larvae by genetic- and chemical-induced ER stress. The numbers in each picture indicate the larvae exhibiting strong bip expression in the liver (open arrowheads)/tested larvae (A) or strong gstp1 expression in the gills (closed arrowheads)/tested larvae (DG). (A) bip expression in WT sibling and pmm2it768 larvae. (B) The expression of bip and chop was analyzed by RT-PCR in WT, pmm2it768/+ (m/+), or pmm2it768/it768 (m/m) larvae. The amount of cDNA used for RT-PCR was standardized by the ef1α expression. (C) The relative expression levels of bip and chop to ef1α was evaluated by qPCR in WT, pmm2it768/+ (m/+), or pmm2it768/it768 (m/m) larvae. a and b indicate statistically significant differences (Tukey’s test, P < 0.001; n = 3 for each genotype). (D) Induced expression of gstp1 after 12-h treatment of 5 μg/mL tunicamycin (TM) or 2 μM thapsigargin (TG) in WT AB larvae injected or not with 1 pmol nrf2aMO. (E) Induced expression of gstp1 after 12-h treatment of 1 μM thapsigargin (TG) in WT sibling and nrf2afh318 larvae. (F) gstp1 expression in WT sibling and pmm2it768 larvae injected or not with 1 pmol perkMO. (G) gstp1 expression in WT sibling and perkit312 larvae injected or not with 1 pmol pmm2MO. The graph shows the percentages of gstp1-positive larvae of the indicated genotypes. The numbers of larvae tested are indicated above the bars. Asterisks denote statistical significance (Fisher’s exact test; *P < 0.001). The data were obtained based on the results of the WISH analysis shown in the Upper panels.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
ddit3 WT standard conditions Day 5 whole organism RTPCR
pmm2it768/+ standard conditions Day 5 whole organism RTPCR
pmm2it768/it768 standard conditions Day 5 whole organism RTPCR
gstp1.2 AB control Day 5 Not Detected gill ISH
AB chemical treatment: thapsigargin Day 5 gill ISH
AB chemical treatment: tunicamycin Day 5 gill ISH
AB + MO1-nfe2l2a control Day 5 Not Detected gill ISH
AB + MO1-nfe2l2a chemical treatment: thapsigargin Day 5 gill ISH
AB + MO1-nfe2l2a chemical treatment: tunicamycin Day 5 gill ISH
WT + MO3-pmm2 standard conditions Day 5 gill ISH
eif2ak3it312/+ standard conditions Day 5 Not Detected gill ISH
eif2ak3it312/+ + MO3-pmm2 standard conditions Day 5 gill ISH
eif2ak3it312/it312 + MO3-pmm2 standard conditions Day 5 gill ISH
nfe2l2afh318/fh318 control Day 5 Not Detected gill ISH
nfe2l2afh318/fh318 chemical treatment: thapsigargin Day 5 Not Detected gill ISH
pmm2it768/it768 control Day 5 gill ISH
pmm2it768/it768 + MO1-nfe2l2a standard conditions Day 5 Not Detected gill ISH
hspa5 WT standard conditions Day 5 whole organism RTPCR
pmm2it768/+ standard conditions Day 5 whole organism RTPCR
pmm2it768/it768 standard conditions Day 5 liver ISH
Day 5 whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB chemical treatment: thapsigargin Day 5 gill gstp1.2 expression increased amount, abnormal
AB chemical treatment: tunicamycin Day 5 gill gstp1.2 expression increased amount, abnormal
AB + MO1-nfe2l2a chemical treatment: thapsigargin Day 5 gill gstp1.2 expression amount, ameliorated
AB + MO1-nfe2l2a chemical treatment: tunicamycin Day 5 gill gstp1.2 expression amount, ameliorated
WT + MO3-pmm2 standard conditions Day 5 gill gstp1.2 expression increased amount, abnormal
eif2ak3it312/+ + MO3-pmm2 standard conditions Day 5 gill gstp1.2 expression increased amount, abnormal
eif2ak3it312/it312 + MO3-pmm2 standard conditions Day 5 gill gstp1.2 expression amount, ameliorated
pmm2it768/it768 control Day 5 gill gstp1.2 expression increased amount, abnormal
pmm2it768/it768 standard conditions Day 5 liver hspa5 expression increased amount, abnormal
Day 5 response to endoplasmic reticulum stress increased occurrence, abnormal
Day 5 whole organism ddit3 expression increased amount, abnormal
Day 5 whole organism hspa5 expression increased amount, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA