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ZFIN ID: ZDB-FIG-180503-3
Pelliccia et al., 2017 - Gdf3 is required for robust Nodal signaling during germ layer formation and left-right patterning. eLIFE   6 Full text @ Elife
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 30%-epiboly to Shield
PHENOTYPE:
Fish:
Observed In:
Stage Range: 30%-epiboly to Shield

Fig. 2

Gdf3 is necessary for the expression of Nodal target genes.

(A–K) RNA in situ hybridization of Nodal target gene expression at 30% epiboly. (A–C) lefty1 (lft1) is normally expressed in the margin (A), but is lost in maternal-zygotic gdf3 mutant embryos (MZgdf3, B) and heterozygous embryos derived from maternal homozygotes (Mgdf3, C). (D–F) cyclops (cyc) is normally expressed in the margin (D). Expression of cyc is reduced in MZgdf3 (E) and Mgdf3 (F) embryos. (G–I) goosecoid (gsc) is normally expressed in the dorsal region (G), but is absent in MZgdf3 (H) and Mgdf3 (I) embryos. (J–K) floating head (flh) is normally expressed in the dorsal region (J) but is diminished in MZgdf3 (K) embryos. (L–Q) RNA in situ hybridization of Nodal target gene expression at the shield stage. (L–M) no tail (ntla) is expressed along the margin in WT embryos (L), but is absent from the dorsal end of MZgdf3 embryos (M, arrow). (N, O) axial (axl) is expressed in the dorsal region of WT embryos (N) but absent in MZgdf3 embryos (O). (P, Q) sox17 is expressed in the margin of WT embryos (P) but absent in MZgdf3 embryos (Q). (R–S) Loss of gsc in MZgdf3 embryos (R) can be rescued by injection of 50 pg gdf3 mRNA. All views are animal, with dorsal to the right and ventral to the left,unless otherwise indicated.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
foxa2 WT standard conditions Shield whole organism dorsal region ISH
gdf3pr05/pr05 standard conditions Shield Not Detected whole organism dorsal region ISH
gsc gdf3pr05/+ standard conditions 30%-epiboly Not Detected whole organism dorsal region ISH
gdf3pr05/+ standard conditions 30%-epiboly whole organism dorsal region ISH
gdf3pr05/pr05 standard conditions 30%-epiboly Not Detected whole organism dorsal region ISH
lft1 gdf3pr05/+ standard conditions 30%-epiboly Not Detected margin ISH
gdf3pr05/+ standard conditions 30%-epiboly margin ISH
gdf3pr05/pr05 standard conditions 30%-epiboly Not Detected margin ISH
ndr2 gdf3pr05/+ standard conditions 30%-epiboly Not Detected margin ISH
gdf3pr05/+ standard conditions 30%-epiboly margin ISH
gdf3pr05/pr05 standard conditions 30%-epiboly Not Detected margin ISH
noto WT standard conditions 30%-epiboly whole organism dorsal region ISH
gdf3pr05/pr05 standard conditions 30%-epiboly Not Detected whole organism dorsal region ISH
sox17 WT standard conditions Shield margin ISH
gdf3pr05/pr05 standard conditions Shield Not Detected margin ISH
tbxta WT standard conditions Shield margin ISH
gdf3pr05/pr05 standard conditions Shield margin ISH
Shield Not Detected margin dorsal region ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
gdf3pr05/+ standard conditions 30%-epiboly margin lft1 expression absent, abnormal
30%-epiboly margin ndr2 expression absent, abnormal
30%-epiboly whole organism dorsal region gsc expression absent, abnormal
gdf3pr05/pr05 standard conditions 30%-epiboly margin ndr2 expression absent, abnormal
30%-epiboly margin lft1 expression absent, abnormal
30%-epiboly whole organism dorsal region noto expression absent, abnormal
30%-epiboly whole organism dorsal region gsc expression absent, abnormal
Shield margin sox17 expression absent, abnormal
Shield margin dorsal region tbxta expression absent, abnormal
Shield whole organism dorsal region foxa2 expression absent, abnormal
Acknowledgments:
ZFIN wishes to thank the journal eLIFE for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Elife