header logo image header logo text
Downloads Login
Research
General Information
ZIRC
ZFIN ID: ZDB-FIG-180502-8
Singh et al., 2017 - Neuropeptide Y Regulates Sleep by Modulating Noradrenergic Signaling. Current biology : CB   27(24):3796-3811.e5 Full text @ Curr. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage: Day 5

Fig. 5

Evidence for Anatomical Interaction between Hindbrain NPY Neurons and the LC

(A–B″) Double FISH using probes specific for npy (A) and dbh (A′) show their close proximity in the LC (A″). Boxed region in (A″) is magnified in a 50-μm-thick maximum-intensity projection in (B–B″).

(C–D′) Tg(npy:kalta4);Tg(uas:nfsb-mcherry);Tg(dbh:EGFP) brains labeled using anti-DsRed (C) and anti-EGFP antibodies (C′). Boxed region in (C″) is magnified 25× in (D) and 63× in (D′). Maximum-intensity projections 40 and 63 μm thick are shown in (D) and (D′).

(E) Surface rendering of the boxed region in (D′).

(F–H) Magnification of the boxed regions in (E). White asterisks show the close proximity of NPY and LC neuron projections.

(I and J) FISH using npy1r- and npy2rl-specific probes and immunostaining using an anti-EGFP antibody in Tg(dbh:EGFP) brains reveal the close proximity of npy1r (I) and npy2rl (J) to dbh-expressing LC neurons. (I′) and (J′) show orthogonal views of the 24- and 25-μm-thick maximum-intensity projections shown in (I) and (J).

a, anterior; p, posterior. Samples are 5-dpf brains. Scale bars, (A″ and C″) 50 μm, (B″ and D) 10 μm, (D′) 7.5 μm, (E) 2.0 μm, (F–H) 0.5 μm, and (I–J) 10 μm.

See also Movie S2.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
dbh AB/TL standard conditions Day 5 locus coeruleus noradrenergic neuron ISH
EGFP ct821Tg(AB/TL) standard conditions Day 5 locus coeruleus noradrenergic neuron IHC
ct852Tg ; c264Tg ; ct821Tg(AB/TL) standard conditions Day 5 locus coeruleus noradrenergic neuron IHC
mCherry ct852Tg ; c264Tg ; ct821Tg(AB/TL) standard conditions Day 5 brain peptidergic neuron IHC
npy AB/TL standard conditions Day 5 brain peptidergic neuron ISH
Day 5 Not Detected locus coeruleus ISH
npy1r ct821Tg(AB/TL) standard conditions Day 5 Not Detected locus coeruleus ISH
npy2rl ct821Tg(AB/TL) standard conditions Day 5 Not Detected locus coeruleus ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Current biology : CB for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Curr. Biol.