ZFIN ID: ZDB-FIG-180622-61
Singh et al., 2017 - Neuropeptide Y Regulates Sleep by Modulating Noradrenergic Signaling. Current biology : CB   27(24):3796-3811.e5 Full text @ Curr. Biol.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Conditions:
Observed In:
Stage: Day 5

Fig. 6 Functional Evidence that NPY Promotes Sleep by Inhibiting NE Signaling (A–D) Tg(hsp:npy);dbh−/− and dbh−/− animals were less active (A and B) and slept more (C and D) than dbh+/− siblings during the day before and after HS. Tg(hsp:npy);dbh+/− animals were less active and slept more than dbh+/− siblings during the day after HS. NPY overexpression in Tg(hsp:npy);dbh−/− animals did not further decrease locomotor activity or increase sleep compared to dbh−/− siblings. Yellow bars indicate HS. Pre-HS and post-HS quantify data on day 5 before and after HS. (E–J) npy+/+, npy+/−, and npy−/− siblings were treated with either DMSO or prazosin. DMSO-treated npy−/− animals were more active (E and F) and slept less (H and I) than their DMSO-treated npy+/− and npy+/+ siblings during the day, but there was no difference at night (E, G, H and J). Prazosin decreased activity (E and F) and increased sleep (H and I) to a similar extent for npy−/−, npy+/−, and npy+/+ siblings during the day. Arrows indicate behavioral artifacts due to addition of water. Mean ± SEM for 2 (A–D) or 4 (E–J) experiments is shown. n.s., not significant; ∗p < 0.05; ∗∗p < 0.01; ∗∗∗∗p < 0.0001 by two-way ANOVA with Holm-Sidak test. See also Figures S6 and S7 and Table S2.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB/TL chemical treatment by environment: prazosin Day 5 circadian sleep/wake cycle, sleep increased occurrence, abnormal
Day 5 swimming decreased occurrence, abnormal
ct853Tg ; dbhct806/ct806(AB/TL) standard conditions Day 5 circadian sleep/wake cycle, sleep increased occurrence, abnormal
Day 5 swimming decreased occurrence, abnormal
ct853Tg ; dbhct806/ct806(AB/TL) heat shock Day 5 circadian sleep/wake cycle, sleep increased occurrence, abnormal
Day 5 swimming decreased occurrence, abnormal
dbhct806/+(AB/TL) standard conditions Day 5 circadian sleep/wake cycle, sleep duration, normal
Day 5 circadian sleep/wake cycle, wakefulness duration, normal
Day 5 swimming occurrence, normal
dbhct806/+(AB/TL) heat shock Day 5 circadian sleep/wake cycle, sleep duration, normal
Day 5 circadian sleep/wake cycle, wakefulness duration, normal
Day 5 swimming occurrence, normal
dbhct806/+ ; ct853Tg(AB/TL) standard conditions Day 5 circadian sleep/wake cycle, sleep duration, normal
Day 5 circadian sleep/wake cycle, wakefulness duration, normal
Day 5 swimming occurrence, normal
dbhct806/+ ; ct853Tg(AB/TL) heat shock Day 5 circadian sleep/wake cycle, sleep increased occurrence, abnormal
Day 5 swimming decreased occurrence, abnormal
dbhct806/ct806(AB/TL) standard conditions Day 5 circadian sleep/wake cycle, sleep increased occurrence, abnormal
Day 5 swimming decreased occurrence, abnormal
dbhct806/ct806(AB/TL) heat shock Day 5 circadian sleep/wake cycle, sleep increased occurrence, abnormal
Day 5 swimming decreased occurrence, abnormal
npyct811/+(AB/TL) control Day 5 circadian sleep/wake cycle, sleep duration, normal
Day 5 swimming occurrence, normal
npyct811/+(AB/TL) chemical treatment by environment: prazosin Day 5 circadian sleep/wake cycle, sleep increased occurrence, abnormal
Day 5 swimming decreased occurrence, abnormal
npyct811/ct811(AB/TL) control Day 5 circadian sleep/wake cycle, sleep decreased duration, abnormal
Day 5 swimming increased occurrence, abnormal
npyct811/ct811(AB/TL) chemical treatment by environment: prazosin Day 5 circadian sleep/wake cycle, sleep increased occurrence, abnormal
Day 5 swimming decreased occurrence, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Current biology : CB for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Curr. Biol.