FIGURE

Fig. 4

ID

ZDB-FIG-180502-7

Publication

Singh et al., 2017 - Neuropeptide Y Regulates Sleep by Modulating Noradrenergic Signaling

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Fig. 4

Loss of npy-Expressing Neurons Reduces Daytime Sleep

(A and A′) Ventral views of brains from 5-dpf Tg(npy:kalta4);Tg(uas:nfsb-mcherry) animals stained with anti-DsRed antibody following treatment with DMSO (A) or 10 mM MTZ (A′), showing nearly complete loss of mCherry after MTZ treatment. Scale bar, 100 μm.

(B) Mean ± SEM mCherry fluorescence intensity for Tg(npy:kalta4);Tg(uas:nfsb-mcherry) animals treated with DMSO (n = 4) or MTZ (n = 4).

(C–N) Tg(npy:kalta4);Tg(uas:nfsb-mcherry) animals treated with MTZ were more active (C and D) and slept less (F and G) than identically treated Tg(npy:kalta4) siblings during the day, due to fewer sleep bouts (I) and longer wake bouts (M). There was no difference in activity (C and E), sleep (F and H), sleep bout number (J) or wake bout length (N) at night, or in sleep bout length during the day (K) or night (L).

Mean ± SEM from three experiments is shown. n, number of animals. ^∗p < 0.05; ^∗∗p < 0.01; ^∗∗∗∗p < 0.0001 by two-tailed Student’s t test.

See also Figures S4 and S5 and Table S1.

Expression Data

Gene:	mCherry
Fish:	c264Tg; ct852Tg (AB/TL)
Condition:	chemical treatment by environment: metronidazole
Anatomical Term:	brain peptidergic neuron
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ct852Tg (AB/TL) c264Tg; ct852Tg (AB/TL)
Condition:	chemical treatment by environment: metronidazole
Observed In:	brain brain peptidergic neuron circadian sleep/wake cycle, sleep circadian sleep/wake cycle, wakefulness peptidergic neuron swimming
Stage:	Day 5

Phenotype Detail

Acknowledgments

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