PUBLICATION
Neuropeptide Y Regulates Sleep by Modulating Noradrenergic Signaling
- Authors
- Singh, C., Rihel, J., Prober, D.A.
- ID
- ZDB-PUB-171212-4
- Date
- 2017
- Source
- Current biology : CB 27(24): 3796-3811.e5 (Journal)
- Registered Authors
- Prober, David, Rihel, Jason
- Keywords
- arousal, genetics, hypothalamus, locomotor activity, locus coeruleus, neuropeptide Y, noradrenaline, sleep, zebrafish
- MeSH Terms
-
- Adrenergic Neurons/metabolism*
- Animals
- Fish Proteins/metabolism*
- Neuropeptide Y/metabolism*
- Signal Transduction*
- Sleep/genetics
- Sleep/physiology*
- Wakefulness/genetics
- Wakefulness/physiology*
- Zebrafish/genetics
- Zebrafish/physiology*
- PubMed
- 29225025 Full text @ Curr. Biol.
Citation
Singh, C., Rihel, J., Prober, D.A. (2017) Neuropeptide Y Regulates Sleep by Modulating Noradrenergic Signaling. Current biology : CB. 27(24):3796-3811.e5.
Abstract
Sleep is an essential and evolutionarily conserved behavioral state whose regulation remains poorly understood. To identify genes that regulate vertebrate sleep, we recently performed a genetic screen in zebrafish, and here we report the identification of neuropeptide Y (NPY) as both necessary for normal daytime sleep duration and sufficient to promote sleep. We show that overexpression of NPY increases sleep, whereas mutation of npy or ablation of npy-expressing neurons decreases sleep. By analyzing sleep architecture, we show that NPY regulates sleep primarily by modulating the length of wake bouts. To determine how NPY regulates sleep, we tested for interactions with several systems known to regulate sleep, and provide anatomical, molecular, genetic, and pharmacological evidence that NPY promotes sleep by inhibiting noradrenergic signaling. These data establish NPY as an important vertebrate sleep/wake regulator and link NPY signaling to an established arousal-promoting system.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping