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ZFIN ID: ZDB-FIG-180502-9
Singh et al., 2017 - Neuropeptide Y Regulates Sleep by Modulating Noradrenergic Signaling. Current biology : CB   27(24):3796-3811.e5 Full text @ Curr. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Conditions:
Anatomical Terms:
Stage: Day 5
PHENOTYPE:
Fish:
Conditions:
Observed In:
Stage: Day 5

Fig. 7

NPY Signaling Affects dbh mRNA Level in the LC

(A–C) ISH showing dbh expression in the LC (A, boxed) and medulla oblongata. dbh mRNA levels were lower in Tg(hsp:npy) animals (A″) compared to WT siblings (A′) after HS. ISH using probes specific for adcyap1a (B) and hcrt (C). Boxed regions in (A)–(C) are quantified in (D) and (E).

(D) dbh mRNA level in the LC is lower in Tg(hsp:npy) animals than WT siblings at 3 hr post-HS, but there is no significant difference at 1, 2, or 7 hr post-HS.

(E) Overexpression of Prok2 or treatment with 20 μM melatonin had no effect on dbh expression. NPY overexpression did not affect adcyap1a or hcrt expression.

(F and F′) dbh mRNA level in the LC was higher in npy−/− animals (F′) compared to npy+/+ siblings (F).

(G) Quantification of dbh mRNA level in the LC of npy−/− animals and sibling controls.

(H) dbh mRNA level in the LC of WT animals was lower at night than during the day.

Mean ± SEM fluorescence intensity from 8–12 brains for each condition is shown. p < 0.05 by two-tailed Student’s t test (D and H) or one-way ANOVA with Holm-Sidak test (G). Samples are 5-dpf brains. Scale bars, (A–C) 100 μm and (A′, A″, F, and F′) 10 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
adcyap1a AB/TL standard conditions Day 5 brain peptidergic neuron ISH
AB/TL heat shock Day 5 brain peptidergic neuron ISH
ct853Tg(AB/TL) heat shock Day 5 brain peptidergic neuron ISH
dbh AB/TL standard conditions Day 5 locus coeruleus noradrenergic neuron ISH
Day 5 medulla oblongata noradrenergic neuron ISH
AB/TL chemical treatment by environment: melatonin Day 5 locus coeruleus noradrenergic neuron ISH
AB/TL heat shock Day 5 locus coeruleus noradrenergic neuron ISH
Day 5 medulla oblongata noradrenergic neuron ISH
ct841Tg(AB/TL) heat shock Day 5 locus coeruleus noradrenergic neuron ISH
ct853Tg(AB/TL) heat shock Day 5 locus coeruleus noradrenergic neuron ISH
Day 5 medulla oblongata noradrenergic neuron ISH
npyct811/+(AB/TL) standard conditions Day 5 locus coeruleus noradrenergic neuron ISH
npyct811/ct811(AB/TL) standard conditions Day 5 locus coeruleus noradrenergic neuron ISH
hcrt AB/TL standard conditions Day 5 brain hypocretin-secreting neuron ISH
AB/TL heat shock Day 5 brain hypocretin-secreting neuron ISH
ct853Tg(AB/TL) heat shock Day 5 brain hypocretin-secreting neuron ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
ct853Tg(AB/TL) heat shock Day 5 locus coeruleus noradrenergic neuron dbh expression decreased amount, abnormal
npyct811/ct811(AB/TL) standard conditions Day 5 locus coeruleus noradrenergic neuron dbh expression increased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Current biology : CB for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Curr. Biol.