NPY Signaling Affects dbh mRNA Level in the LC
(A–C) ISH showing dbh expression in the LC (A, boxed) and medulla oblongata. dbh mRNA levels were lower in Tg(hsp:npy) animals (A″) compared to WT siblings (A′) after HS. ISH using probes specific for adcyap1a (B) and hcrt (C). Boxed regions in (A)–(C) are quantified in (D) and (E).
(D) dbh mRNA level in the LC is lower in Tg(hsp:npy) animals than WT siblings at 3 hr post-HS, but there is no significant difference at 1, 2, or 7 hr post-HS.
(E) Overexpression of Prok2 or treatment with 20 μM melatonin had no effect on dbh expression. NPY overexpression did not affect adcyap1a or hcrt expression.
(F and F′) dbh mRNA level in the LC was higher in npy−/− animals (F′) compared to npy+/+ siblings (F).
(G) Quantification of dbh mRNA level in the LC of npy−/− animals and sibling controls.
(H) dbh mRNA level in the LC of WT animals was lower at night than during the day.
Mean ± SEM fluorescence intensity from 8–12 brains for each condition is shown. ∗p < 0.05 by two-tailed Student’s t test (D and H) or one-way ANOVA with Holm-Sidak test (G). Samples are 5-dpf brains. Scale bars, (A–C) 100 μm and (A′, A″, F, and F′) 10 μm.