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ZIRC
ZFIN ID: ZDB-FIG-180207-6
Choi et al., 2018 - Targeted knockout of a chemokine-like gene increases anxiety and fear responses. Proceedings of the National Academy of Sciences of the United States of America   115(5):E1041-E1050 Full text @ Proc. Natl. Acad. Sci. USA
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Adult

Fig. 1

Characterization of sam2-expressing cells in the adult zebrafish brain. (A and A′) Whole-mount two color in situ hybridization of a dissected zebrafish brain with sam2 and a dopaminergic neuron marker, th. Anterior is to the left; lateral (A) and dorsal view (A′). The sam2 expression region did not overlap with th+ neurons. Prominent sam2 expression is seen in the Vd and Hb as well as hypothalamic regions. (BD) Section images of brain hybridized with sam2/vglut2b (B, sagittal section; C, cross-section) or sam2/gad1b (D). (E and F) Cross-section of Hb region stained with sam2 alone (E) or sam2/vglut2a (F) probes. AN, auditory nerve; D, area dorsalis telencephlali; Dc, central zone of area dorsalis telencephali; MO, medulla oblongata; OB, olfactory bulb; TeO, optic tectum; Vv, ventral nucleus of area ventral telencephali. (Scale bars, 100 µm.)

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
gad1b WT standard conditions Adult central nucleus of ventral telencephalon ISH
Adult dorsal nucleus of ventral telencephalon ISH
sam2 WT standard conditions Adult caudal periventricular hypothalamus ISH
Adult caudal tuberal nucleus ISH
Adult dorsal habenular nucleus ISH
Adult dorsal nucleus of ventral telencephalon ISH
Adult dorsal periventricular hypothalamus ISH
Adult hypothalamus ISH
Adult inner ear neuron ISH
Adult medial zone of dorsal telencephalon ISH
Adult rostral tuberal nucleus ISH
Adult telencephalon ISH
slc17a6a WT standard conditions Adult caudal tuberal nucleus ISH
Adult rostral tuberal nucleus ISH
th WT standard conditions Adult brain dopaminergic neuron ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Proceedings of the National Academy of Sciences of the United States of America for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Proc. Natl. Acad. Sci. USA