Fig. S2

Conserved synteny and CNS-specific expression of samdori gene family. (A) Alignments of zebrafish sam1a and sam1b amino acids with that of human SAM1 and mouse Sam1 (Genbank accession for human and mouse, NM_213609; NM_182808, respectively). (B) Alignments of zebrafish sam2 amino acids with human SAM2 and mouse Sam2 (NM_178539; NM_182807). (C) Alignments of zebrafish sam3a and sam3b amino acids with human SAM3 and mouse Sam3 (NM_182759; NM_183224). (D) Alignments of zebrafish sam4 amino acid with human SAM4 and mouse Sam4 (NM_001005527; NM_177233). (E) Alignments of zebrafish sam5a and sam5b amino acids with human SAM5.1, SAM5.2 and mouse Sam5.1, Sam5.2 (NM_001082967; NM_015381; NM_001252310; NM_134096). (F-O) Whole-mount in situ hybridization was performed to detect the expression of mRNAs. All sam gene family members are exclusively expressed in the brain. Three day-old (3d) embryos (F-M’). sam3a and sam3b showed additional expression in the spinal cord at 24 h, lateral views (N, O). Lateral views (F-M). Dorsal views (F’-M’). Scale bar, 100 μm.