FIGURE

Fig. S1

ID

ZDB-FIG-180207-9

Publication

Choi et al., 2018 - Targeted knockout of a chemokine-like gene increases anxiety and fear responses

Other Figures

All Figure Page

Back to All Figure Page

Fig. S1

Dual reporter system used for insertional mutagenesis, the golden fish project. (A) Body color change in MCH-expressing transgenic zebrafish (TG, a cmv:mch tg line), compared to wild-type adult fish (WT). (B) Expression of dual reporter, GFP-MCH fusion protein, in ef1a:gfp-mch-injected zebrafish embryos. GFP fluorescence and melanosome aggregation are detectable in injected embryos, inj (+), compared to un-injected control embryo, inj (-). (C) Structure of a gene trap vector used in insertional mutagenesis. IR/DR; inverted repeat/direct repeat element; ef1a, elongation factor 1 alpha (ef1a) gene promoter; sp, signal peptide; GFP; green fluorescent protein; MCH, melanin-concentrating hormone; SD, splice donor. (D) Representative mutants showing GFP expression in various tissues (ExTSD1-7; images not to scale). (E) Mapping of the insertion site (arrow) in the intron 3 of a samdori gene on chromosome 4.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA