ZFIN ID: ZDB-FIG-180104-34
He et al., 2017 - Chemical biology reveals CARF as a positive regulator of canonical Wnt signaling by promoting TCF/β-catenin transcriptional activity. Cell discovery   3:17003 Full text @ Cell Discov
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: 2-cell to Day 4
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Germ-ring to Prim-15

Fig. 4

CARF facilitates Wnt/β-catenin signaling in zebrafish embryogenesis. (a) The whole mount in situ analysis of CARF expression in zebrafish embryogenesis. The embryos at indicated developmental stages were fixed for in situ hybridization with CARF probe. (b) CARF knockdown attenuates Wnt activation in Tg(tcf:egfp) line. (c, d) CARFcas009 mutant or CARF morphants show reduced Wnt signaling activity via a p53-independet manner. Embryos injected with the indicated morpholinos or morpholino-mRNA mixture were fixed at the 60% epiboly stage and then analyzed by WISH with cdx4 (left) or tbx6 (right) probe. The relative expression of each marker was classified into two categories: normal (N) or weak (W). The number of the total embryos scored (n) is shown on the top of each bar.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
cdkn2aip TU control 2-cell whole organism ISH
64-cell whole organism ISH
1k-cell whole organism ISH
Shield whole organism ISH
1-4 somites whole organism ISH
14-19 somites whole organism ISH
Prim-5 whole organism ISH
Prim-25 whole organism ISH
Long-pec whole organism ISH
Protruding-mouth whole organism ISH
Day 4 whole organism ISH
cdx4 TU control Germ-ring to Shield presumptive ventral mesoderm ISH
TU + MO1-cdkn2aip control Germ-ring to Shield presumptive ventral mesoderm ISH
TU + MO1-cdkn2aip + MO1-wnt8a + MO2-wnt8a control Germ-ring to Shield presumptive ventral mesoderm ISH
TU + MO1-cdkn2aip + MO1-wnt8a + MO2-wnt8a + MO7-tp53 control Germ-ring to Shield presumptive ventral mesoderm ISH
TU + MO1-wnt8a + MO2-wnt8a control Germ-ring to Shield presumptive ventral mesoderm ISH
cdkn2aipcas009/cas009 control Germ-ring to Shield presumptive ventral mesoderm ISH
cdkn2aipcas009/cas009 + MO1-wnt8a + MO2-wnt8a control Germ-ring to Shield presumptive ventral mesoderm ISH
cdkn2aipcas009/cas009 + MO1-wnt8a + MO2-wnt8a + MO7-tp53 control Germ-ring to Shield presumptive ventral mesoderm ISH
GFP ia4Tg control Prim-15 whole organism IFL
ia4Tg + MO1-cdkn2aip control Prim-15 whole organism IFL
tbx6 TU control Germ-ring to Shield presumptive ventral mesoderm ISH
TU + MO1-cdkn2aip control Germ-ring to Shield presumptive ventral mesoderm ISH
TU + MO1-cdkn2aip + MO1-wnt8a + MO2-wnt8a control Germ-ring to Shield presumptive ventral mesoderm ISH
TU + MO1-cdkn2aip + MO1-wnt8a + MO2-wnt8a + MO7-tp53 control Germ-ring to Shield presumptive ventral mesoderm ISH
TU + MO1-wnt8a + MO2-wnt8a control Germ-ring to Shield presumptive ventral mesoderm ISH
cdkn2aipcas009/cas009 control Germ-ring to Shield presumptive ventral mesoderm ISH
cdkn2aipcas009/cas009 + MO1-wnt8a + MO2-wnt8a control Germ-ring to Shield presumptive ventral mesoderm ISH
cdkn2aipcas009/cas009 + MO1-wnt8a + MO2-wnt8a + MO7-tp53 control Germ-ring to Shield presumptive ventral mesoderm ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
TU + MO1-cdkn2aip control Germ-ring - Shield canonical Wnt signaling pathway decreased process quality, abnormal
Germ-ring - Shield presumptive ventral mesoderm cdx4 expression decreased amount, abnormal
Germ-ring - Shield presumptive ventral mesoderm tbx6 expression decreased amount, abnormal
TU + MO1-cdkn2aip + MO1-wnt8a + MO2-wnt8a control Germ-ring - Shield canonical Wnt signaling pathway decreased process quality, abnormal
Germ-ring - Shield presumptive ventral mesoderm cdx4 expression decreased amount, abnormal
Germ-ring - Shield presumptive ventral mesoderm tbx6 expression decreased amount, abnormal
TU + MO1-cdkn2aip + MO1-wnt8a + MO2-wnt8a + MO7-tp53 control Germ-ring - Shield canonical Wnt signaling pathway decreased process quality, abnormal
Germ-ring - Shield presumptive ventral mesoderm cdx4 expression decreased amount, abnormal
Germ-ring - Shield presumptive ventral mesoderm tbx6 expression decreased amount, abnormal
TU + MO1-wnt8a + MO2-wnt8a control Germ-ring - Shield canonical Wnt signaling pathway decreased process quality, abnormal
Germ-ring - Shield presumptive ventral mesoderm cdx4 expression decreased amount, abnormal
Germ-ring - Shield presumptive ventral mesoderm tbx6 expression decreased amount, abnormal
cdkn2aipcas009/cas009 control Germ-ring - Shield canonical Wnt signaling pathway decreased process quality, abnormal
Germ-ring - Shield presumptive ventral mesoderm cdx4 expression decreased amount, abnormal
Germ-ring - Shield presumptive ventral mesoderm tbx6 expression decreased amount, abnormal
cdkn2aipcas009/cas009 + MO1-wnt8a + MO2-wnt8a control Germ-ring - Shield canonical Wnt signaling pathway decreased process quality, abnormal
Germ-ring - Shield presumptive ventral mesoderm cdx4 expression decreased amount, abnormal
Germ-ring - Shield presumptive ventral mesoderm tbx6 expression decreased amount, abnormal
cdkn2aipcas009/cas009 + MO1-wnt8a + MO2-wnt8a + MO7-tp53 control Germ-ring - Shield canonical Wnt signaling pathway decreased process quality, abnormal
Germ-ring - Shield presumptive ventral mesoderm cdx4 expression decreased amount, abnormal
Germ-ring - Shield presumptive ventral mesoderm tbx6 expression decreased amount, abnormal
ia4Tg + MO1-cdkn2aip control Prim-15 canonical Wnt signaling pathway decreased process quality, abnormal
Prim-15 whole organism GFP expression decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Cell discovery for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Cell Discov