FIGURE

Fig. 4

ID
ZDB-FIG-180104-34
Publication
He et al., 2017 - Chemical biology reveals CARF as a positive regulator of canonical Wnt signaling by promoting TCF/β-catenin transcriptional activity
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Fig. 4

CARF facilitates Wnt/β-catenin signaling in zebrafish embryogenesis. (a) The whole mount in situ analysis of CARF expression in zebrafish embryogenesis. The embryos at indicated developmental stages were fixed for in situ hybridization with CARF probe. (b) CARF knockdown attenuates Wnt activation in Tg(tcf:egfp) line. (c, d) CARFcas009 mutant or CARF morphants show reduced Wnt signaling activity via a p53-independet manner. Embryos injected with the indicated morpholinos or morpholino-mRNA mixture were fixed at the 60% epiboly stage and then analyzed by WISH with cdx4 (left) or tbx6 (right) probe. The relative expression of each marker was classified into two categories: normal (N) or weak (W). The number of the total embryos scored (n) is shown on the top of each bar.
Expression Data
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: 2-cell to Day 4

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Germ-ring to Prim-15

Phenotype Detail
Acknowledgments
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