Fig. 2
- ID
- ZDB-FIG-180104-19
- Publication
- Sandoval et al., 2017 - A metabolic switch controls intestinal differentiation downstream of Adenomatous polyposis coli (APC).
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Knock down of mpc1 expression phenocopies loss of apc. (A) Gross phenotype associated with mpc1 knock down. (B) Whole mount in situ hybridization analysis for organ-specific markers in mpc1 mo. Alcian blue staining revealed improper cartilage development (*) and confirmed loss of pectoral fins in mpc1 mo. pectoral fin bud (pfb), heart (h), gut (g), pancreas (p). (C) Cross section of the eye and gut from control or mpc1 mo. Prior to sectioning, embryos were previously stained with eye and gut-specific markers, irbp (red arrow) and fabp2 (black arrow), respectively. (D) Co-injection with human MPC1 RNA led to increased percentage of mpc1 mo with normal pectoral fins as determined by in situ staining for idi1. Statistical significance was analyzed using Fisher’s exact test. See also Figure 2—figure supplements 1, 2. |
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| Stage Range: | Long-pec to Day 4 |