header logo image header logo text
Downloads Login
Research
General Information
ZIRC
ZFIN ID: ZDB-FIG-171206-50
Chang et al., 2017 - Ftr82 Is Critical for Vascular Patterning during Zebrafish Development. International Journal of Molecular Sciences   18(1) Full text @ Int. J. Mol. Sci.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Prim-5
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Prim-5

Fig. 6

Knockdown of ftr82 affects the expression of vascular markers. Compared to uninjected controls (A,C,E,G,I), the expression of the pan-vascular markers flk and stabilin at vessels (v) and caudal vein plexus (CVP) were decreased in ftr82 morphants at 24 hpf (AD). In addition, the expression of venous markers flt4 (E,F) and mrc1 (G,H) at posterior cardinal vein (pcv) and arterial marker ephrinb2 at aorta (a), were reduced in ftr82 morphants (I,J). (K) Quantification of the relative expression level by qPCR assay showed reduced expression in vascular markers flk (0.14 ± 0.01), flt4 (0.26 ± 0.02), mrc1 (0.35 ± 0.28), and ephrinb2 (0.58 ± 0.06) in ftr82 morphants. *** refers to p < 0.0001 and ** refers to p < 0.001 by an unpaired Student’s t-test. Data are represented as means ± S.D. Scale bars are 200 μm in AH.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
efnb2a WT control Prim-5 artery ISH
Prim-5 whole organism RTPCR
WT + MO2-ftr82 standard conditions Prim-5 artery ISH
Prim-5 whole organism RTPCR
flt4 WT control Prim-5 caudal vein plexus ISH
Prim-5 intersegmental vessel ISH
Prim-5 posterior cardinal vein ISH
Prim-5 vein ISH
Prim-5 whole organism RTPCR
WT + MO2-ftr82 standard conditions Prim-5 caudal vein plexus ISH
Prim-5 intersegmental vessel ISH
Prim-5 posterior cardinal vein ISH
Prim-5 whole organism RTPCR
kdrl WT control Prim-5 vasculature ISH
Prim-5 whole organism RTPCR
WT + MO2-ftr82 standard conditions Prim-5 vasculature ISH
Prim-5 whole organism RTPCR
mrc1a WT control Prim-5 caudal vein plexus ISH
Prim-5 posterior cardinal vein ISH
Prim-5 whole organism RTPCR
WT + MO2-ftr82 standard conditions Prim-5 caudal vein plexus ISH
Prim-5 posterior cardinal vein ISH
Prim-5 whole organism RTPCR
stab2 WT control Prim-5 vasculature ISH
WT + MO2-ftr82 standard conditions Prim-5 vasculature ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO2-ftr82 standard conditions Prim-5 artery efnb2a expression decreased amount, abnormal
Prim-5 caudal vein plexus flt4 expression decreased amount, abnormal
Prim-5 caudal vein plexus mrc1a expression decreased amount, abnormal
Prim-5 intersegmental vessel flt4 expression decreased amount, abnormal
Prim-5 posterior cardinal vein flt4 expression decreased amount, abnormal
Prim-5 posterior cardinal vein mrc1a expression decreased amount, abnormal
Prim-5 vasculature kdrl expression decreased amount, abnormal
Prim-5 vasculature stab2 expression decreased amount, abnormal
Prim-5 whole organism mrc1a expression decreased amount, abnormal
Prim-5 whole organism efnb2a expression decreased amount, abnormal
Prim-5 whole organism flt4 expression decreased amount, abnormal
Prim-5 whole organism kdrl expression decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal International Journal of Molecular Sciences for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Int. J. Mol. Sci.