Knockdown of ftr82 affects the expression of vascular markers. Compared to uninjected controls (A,C,E,G,I), the expression of the pan-vascular markers flk and stabilin at vessels (v) and caudal vein plexus (CVP) were decreased in ftr82 morphants at 24 hpf (A–D). In addition, the expression of venous markers flt4 (E,F) and mrc1 (G,H) at posterior cardinal vein (pcv) and arterial marker ephrinb2 at aorta (a), were reduced in ftr82 morphants (I,J). (K) Quantification of the relative expression level by qPCR assay showed reduced expression in vascular markers flk (0.14 ± 0.01), flt4 (0.26 ± 0.02), mrc1 (0.35 ± 0.28), and ephrinb2 (0.58 ± 0.06) in ftr82 morphants. *** refers to p < 0.0001 and ** refers to p < 0.001 by an unpaired Student’s t-test. Data are represented as means ± S.D. Scale bars are 200 μm in A–H.