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ZFIN ID: ZDB-FIG-170925-14
He et al., 2017 - Insm1a Is Required for Zebrafish Posterior Lateral Line Development.. Frontiers in molecular neuroscience   10:241 Full text @ Front. Mol. Neurosci.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: Prim-15
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Prim-15

Fig. 6

Insm1a regulates the expression of Wnt and Fgf signaling components. Expression of the Wnt and Fgf target genes lef1 (A,B), axin2 (D,E), fgf3 (G,H), fgf10a (I,J), and pea3 (K,L) in control embryos and in insm1a morphants at 30–32 hpf. (C,F) The ratio of lef1-positive cells (C) or axin2-positive cells (F) to total cells in the primordia of controls (black bars) and insm1a morphants (gray bars). Data are expressed as mean ± s.e.m. ***p < 0.001. In insm1a primordia, the expression domains of lef1 and axin2 expand, whereas fgf3 and fgf10a expression is normal but pea3 is reduced. The primordium is outlined with a dotted line, and the anterior is to the left. Scale bar: 10 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
axin2 WT standard conditions Prim-15 posterior lateral line primordium ISH
WT + MO4-tp53 + MO5-insm1a standard conditions Prim-15 posterior lateral line primordium ISH
etv4 WT standard conditions Prim-15 posterior lateral line primordium ISH
WT + MO4-tp53 + MO5-insm1a standard conditions Prim-15 posterior lateral line primordium ISH
lef1 WT standard conditions Prim-15 posterior lateral line primordium ISH
WT + MO4-tp53 + MO5-insm1a standard conditions Prim-15 posterior lateral line primordium ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO4-tp53 + MO5-insm1a standard conditions Prim-15 posterior lateral line primordium etv4 expression decreased amount, abnormal
Prim-15 posterior lateral line primordium axin2 expression increased distribution, abnormal
Prim-15 posterior lateral line primordium lef1 expression increased distribution, abnormal
Prim-15 regulation of canonical Wnt signaling pathway disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Frontiers in molecular neuroscience for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Front. Mol. Neurosci.