Insm1a regulates the expression of Wnt and Fgf signaling components. Expression of the Wnt and Fgf target genes lef1 (A,B), axin2 (D,E), fgf3 (G,H), fgf10a (I,J), and pea3 (K,L) in control embryos and in insm1a morphants at 30–32 hpf. (C,F) The ratio of lef1-positive cells (C) or axin2-positive cells (F) to total cells in the primordia of controls (black bars) and insm1a morphants (gray bars). Data are expressed as mean ± s.e.m. ***p < 0.001. In insm1a primordia, the expression domains of lef1 and axin2 expand, whereas fgf3 and fgf10a expression is normal but pea3 is reduced. The primordium is outlined with a dotted line, and the anterior is to the left. Scale bar: 10 μm.