ZFIN ID: ZDB-FIG-170825-38
Wehner et al., 2017 - Wnt signaling controls pro-regenerative Collagen XII in functional spinal cord regeneration in zebrafish. Nature communications   8:126 Full text @ Nat. Commun.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Conditions:
Anatomical Terms:
Stage Range: Day 4 to Day 5
PHENOTYPE:
Fish:
Conditions:
Observed In:
Stage Range: Day 4 to Day 5

Fig. 7

Overexpression of col12a1a promotes axon regeneration in Wnt/β-catenin pathway-inhibited and wild type zebrafish larvae. a cerulean mRNA is robustly detectable 24 h after the last heat shock in hs:col12a1a-p2a-Cerulean transgenic larvae. b col12a1a overexpression increases anti-Col XII immunoreactivity in the lesion site of Wnt/β-pathway-inhibited (IWR-1 treatment) hs:col12a1a transgenic larvae (arrow; t-test: ***P < 0.001). c col12a1a overexpression rescues axon regeneration in Wnt/β-catenin pathway-inhibited (IWR-1 treatment) hs:col12a1a transgenic larvae (Fischer’s exact test: ***P < 0.001). d col12a1a overexpression improves functional recovery in Wnt/β-catenin pathway-inhibited (IWR-1 treatment) hs:col12a1a transgenic larvae (t-test: ***P < 0.001). e col12a1a overexpression is sufficient to augment axon regeneration (Fischer’s exact test: *** P< 0.001). ae Views are lateral (dorsal is up, rostral is left). BF: brightfield. Scale bars: 200 µm c and 100 µm ac, e. Error bars indicate s.e.m

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
Cerulean ue104Tg heat shock Day 4 whole organism ISH
DsRed zf148Tg perforation: spinal cord, heat shock, chemical treatment by environment: IWR-1-endo Day 5 regenerating tissue axon IFL
zf148Tg ; ue104Tg perforation: spinal cord, heat shock, chemical treatment by environment: IWR-1-endo Day 5 regenerating tissue axon IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
ue104Tg heat shock Day 4 whole organism Cerulean expression increased amount, abnormal
zf148Tg ; ue104Tg perforation: spinal cord, heat shock, chemical treatment by environment: IWR-1-endo Day 5 spinal cord axon regeneration increased efficacy, abnormal
Day 5 swimming occurrence, ameliorated
Acknowledgments:
ZFIN wishes to thank the journal Nature communications for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Nat. Commun.