ZFIN ID: ZDB-FIG-170703-20
Wei et al., 2017 - The guanine nucleotide exchange factor Net1 facilitates the specification of dorsal cell fates in zebrafish embryos by promoting maternal β-catenin activation. Cell Research   27(2):202-225 Full text @ Cell Res.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: 1-cell to 75%-epiboly
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Sphere to 75%-epiboly

Fig. 1 ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
bmp2b WT control Shield whole organism ventral region ISH
WT + MO1-net1 standard conditions Shield whole organism ventral region ISH
chrd WT control Sphere whole organism dorsal region ISH
Shield shield ISH
WT + MO1-net1 standard conditions Sphere whole organism RTPCR
Sphere whole organism dorsal region ISH
Shield shield ISH
Shield whole organism RTPCR
dharma WT control Sphere whole organism dorsal region ISH
WT + MO1-net1 standard conditions Sphere whole organism RTPCR
Sphere whole organism dorsal region ISH
gata2a WT control 75%-epiboly non neural ectoderm ventral region ISH
WT + MO1-net1 standard conditions 75%-epiboly non neural ectoderm ventral region ISH
gsc WT control Shield shield ISH
WT + MO1-net1 standard conditions Shield shield ISH
Shield whole organism RTPCR
net1 WT standard conditions 1-cell Not Detected whole organism ISH
1-cell Not Detected whole organism RTPCR
64-cell Not Detected whole organism ISH
256-cell Not Detected whole organism RTPCR
1k-cell whole organism RTPCR
30%-epiboly margin dorsal region ISH
30%-epiboly margin dorsal-ventral gradient ISH
Sphere whole organism RTPCR
Shield margin ISH
Shield margin dorsal region ISH
Shield whole organism RTPCR
sox3 WT control 75%-epiboly neuroectoderm dorsal region ISH
WT + MO1-net1 standard conditions 75%-epiboly neuroectoderm dorsal region ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Qualifier Anatomy
Ab1-net1 WB WT standard conditions 256-cell Not Detected whole organism
WB 30%-epiboly whole organism
WB Shield whole organism
WB 75%-epiboly whole organism
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-net1 standard conditions Sphere dorsal/ventral axis specification decreased process quality, abnormal
Sphere whole organism dharma expression decreased amount, abnormal
Sphere whole organism chrd expression decreased amount, abnormal
Sphere whole organism wholly ventralized, abnormal
Sphere whole organism dorsal region dharma expression decreased amount, abnormal
Sphere whole organism dorsal region chrd expression decreased amount, abnormal
Sphere whole organism dorsal region chrd expression decreased distribution, abnormal
Sphere whole organism dorsal region dharma expression decreased distribution, abnormal
Shield dorsal/ventral axis specification decreased process quality, abnormal
Shield shield gsc expression decreased amount, abnormal
Shield shield chrd expression decreased amount, abnormal
Shield shield chrd expression decreased distribution, abnormal
Shield shield gsc expression decreased distribution, abnormal
Shield whole organism gsc expression decreased amount, abnormal
Shield whole organism chrd expression decreased amount, abnormal
Shield whole organism wholly ventralized, abnormal
Shield whole organism ventral region bmp2b expression increased distribution, abnormal
75%-epiboly dorsal/ventral axis specification decreased process quality, abnormal
75%-epiboly neuroectoderm dorsal region sox3 expression decreased amount, abnormal
75%-epiboly neuroectoderm dorsal region sox3 expression decreased distribution, abnormal
75%-epiboly non neural ectoderm ventral region gata2a expression increased distribution, abnormal
75%-epiboly whole organism wholly ventralized, abnormal
Full text @ Cell Res.