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Fig. 3
Effects of wnt8a knockdown and BIO on renal progenitor cell number. A-G) Left panels show flat mounted dorsal views of the trunks of 16 hpf embryos stained for pax2a transcripts and treated as indicated. Right panels show close-up views Hnf1b+ nuclei in the proximal pronephros (location indicated by the red dotted box in the embryo in A). Scale bar represents 50 µm. H) Scatter-graph showing empirical counts of Hnf1b+ nuclei after the various treatments shown.
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Reprinted from Developmental Biology, 425(2), Naylor, R.W., Han, H.I., Hukriede, N.A., Davidson, A.J., Wnt8a expands the pool of embryonic kidney progenitors in zebrafish, 130-141, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.