FIGURE

Fig. S3

ID

ZDB-FIG-170421-9

Publication

Goldman et al., 2017 - Resolving Heart Regeneration by Replacement Histone Profiling

Other Figures

All Figure Page

Back to All Figure Page

Fig. S3

Dynamic H3.3 Enrichment Near Genes with Expression Changes During Regeneration (related to Figure 4)

(A) Genome browser snapshots of uninjured (top track, dark blue) and regenerating (bottom track, dark red) H3.3 profiles are shown around genes previously known to be induced in CMs during regeneration. Pink arrows highlight differential H3.3 peaks. Genes are cartooned at the bottom in blue. Top right, x-axis.

(B) Correlation of changes in RNAseq and changes in H3.3 occupancy (Pearson = 0.33).

(C) Venn diagram showing there are 11,964 intergenic peaks that are detectable only in regenerating CMs versus uninjured CMs. These peaks emerge in the vicinity of 5,232 genes (see Table S2).

(D) Extracellular matrix protein gene angptl2, transcription factor gene csrp1a, and translation elongation factor gene eif4e1c all have increased expression during regeneration. Peaks used to generate reporters are marked by a dashed box (see Figure S4). In situ hybridization signals (violet) appear to reflect increases observed in H3.3 enrichment during regeneration (bottom tracks). Scale bar, 125 μm.

Expression Data

Gene:	EGFP
Fish:	pd186Tg pd188Tg pd190Tg pd192Tg pd194Tg pd196Tg pd198Tg pd200Tg pd202Tg pd204Tg pd206Tg pd208Tg pd212Tg pd214Tg pd216Tg pd218Tg pd220Tg pd224Tg pd226Tg pd228Tg pd230Tg pd232Tg pd234Tg pd236Tg pd238Tg pd242Tg pd244Tg pd246Tg
Anatomical Terms:	caudal fin central nervous system cerebellum diencephalon eye head heart integument neuromast notochord optic tectum pectoral fin fold pericardium peripheral olfactory organ pharyngeal arch spinal cord trunk musculature whole organism
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Cell, 40, Goldman, J.A., Kuzu, G., Lee, N., Karasik, J., Gemberling, M., Foglia, M.J., Karra, R., Dickson, A.L., Sun, F., Tolstorukov, M.Y., Poss, K.D., Resolving Heart Regeneration by Replacement Histone Profiling, 392-404.e5, Copyright (2017) with permission from Elsevier. Full text @ Dev. Cell