Fig. 5

pdgfra influences the movement of the ALPM after the 15 somite stage.

(A–F) Dorsal views, anterior to the top, depict expression of hand2 in the wt (A–C) and ref mutant (D–F) ALPM from 12 to 20 s. The morphology and position of the ALPM are indistinguishable in wt (A) and ref mutant (D) embryos at 12 s. After 15 s (B,C,E,F), disrupted movement of the ALPM is evident in ref mutants. Scale bar: 60 μm. (G) Graph illustrates the average distance between the two sides of the ALPM in wt and ref mutant embryos from 8 to 20 s. In each embryo, the distance between the sides of the ALPM was calculated by measuring the distance between the medial edges of the hand2-expressing domains at three equidistant points (200 μm apart) along the anterior-posterior axis. The largest of those three measurements was selected as representative of the maximum distance between the bilateral ALPM domains for that embryo. Dots represent the selected measurements from individual embryos. The distance between the bilateral sheets in ref mutant embryos begins to diverge significantly from wt after 15 s. Error bars represent the standard error. Asterisks indicate p<0.05 (Student’s t-test): p=0.99 at 8 s; p=0.58 at 12 s; p=0.30 at 14 s; p=0.053 at 15 s; p=0.012 at 16 s; and p=0.00012 at 20 s.