Fig. 5 S2

Overexpression of pdgfaa influences ALPM movement by the 15 somite stage.

(A–C) Dorsal views, anterior to the top, depict expression of hand2 in the ALPM of Tg(hsp70l:pdgfaa-2A-mCherry) embryos from 12 to 20 s, following heat shock at the tailbud stage. See Figure 5A–C for images of representative wt embryos at the same stages. Overexpression of pdgfaa at the tailbud stage does not seem to affect the morphology and position of the ALPM at 12 s (A), but it does disrupt ALPM movement by 16 s (B) and 20 s (C). Scale bar: 60 μm. (D) Graph illustrates the average distance between the two sides of the ALPM from 12 to 20 s in Tg(hsp70l:pdgfaa-2A-mCherry) embryos, following heat shock at the tailbud stage, shown together with the data presented in Figure 5G. The distance between the sides of the ALPM domains in transgenic embryos was measured as described for Figure 5G. Dots represent measurements from individual embryos. The distance between the bilateral sheets of ALPM in Tg(hsp70l:pdgfaa-2A-mCherry) embryos begins to diverge significantly from wt by 15 s. Error bars represent the standard error. Asterisks indicate p<0.05 in comparison to wt embryos (Student’s T-test): p=0.47 at 12 s; p=0.12 at 14 s; p=0.024 at 15 s; p=0.090 at 16 s; and p=0.000027 at 20 s.