FIGURE

Fig. 2

ID

ZDB-FIG-170227-2

Publication

Lessieur et al., 2017 - The Ciliopathy Gene ahi1 Is Required for Zebrafish Cone Photoreceptor Outer Segment Morphogenesis and Survival

Other Figures

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Fig. 2

Loss of ahi1 affects other ciliated tissues. (A, B) Anti-acetylated tubulin whole-mount immunostaining (green) of kidney duct in wild-type and ahi1^lri46 mutants at 36 hpf. Anterior is to the left in both images. Cilia line the pronephric duct (small arrowheads) in wild-type animals. The contralateral duct also can be observed (small arrows). Cilia are absent in the middle and distal pronephros in ahi1^lri46 mutants (arrowhead in [B] indicates the proximal boundary where cilia are missing). (C, D) In the proximal region, mutant cilia are not qualitatively different from wild-type controls. (E, F) Cilia in the distal region of the pronephros are absent in mutant animals when compared to controls. Ift88 immunostaining (red) was used to mark the ciliary transition zone. Scale bars: 50 μm (A, B) and 20 μm (C–F).

Expression Data

Gene:	ift88
Antibodies:	Ab1-ift88 Ab1-tuba
Fish:	WT ahi1^lri46/lri46
Anatomical Terms:	pronephric duct ciliary transition zone pronephric duct motile cilium
Stage:	Prim-25

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ahi1^lri46/lri46
Observed In:	posterior pronephric duct posterior pronephric duct motile cilium pronephric duct cilium assembly
Stage:	Prim-25

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Invest. Ophthalmol. Vis. Sci.