FIGURE

Fig. 1

ID

ZDB-FIG-170227-1

Publication

Lessieur et al., 2017 - The Ciliopathy Gene ahi1 Is Required for Zebrafish Cone Photoreceptor Outer Segment Morphogenesis and Survival

Other Figures

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Fig. 1

Generation of ahi1 mutant zebrafish. (A–C) Whole-mount in situ hybridization from 1 cell (A), 10 hpf (B), and 48 hpf (C), showing expression of ahi1. (D, E) Lateral and ventral views showing 5 dpf zebrafish stained by whole-mount in situ hybridization. ahi1 expression was observed throughout the central nervous system (arrowheads) and pronephros (arrow). (F) A transverse section shows ahi1 expression throughout the retina at 5 dpf. (G) Illustration of ahi1 genomic architecture. The wild-type sequence and TALENs induced ahi1 mutant alleles are shown. Wild-type nucleotides are in upper case. Deleted nucleotides are denoted as dashes while inserted nucleotides are in lower case letters. (H) Schematic protein structure of Ahi1 illustrating the location of the coiled-coil (CC) domain, WD40 repeats, and SH3 domain. The ahi1^lri46 is a frame shift mutation and encodes a truncated protein lacking the 7 WD 40 repeats and the SH3 domain. (I) Chromatograms of Sanger sequencing reactions of ahi1 wild type (wild-type) and homozygous mutant (ahi1^lri46) zebrafish. (J) Lateral view of representative wild-type and ahi1^lri46 homozygous mutant at 5 dpf.

Expression Data

Gene:	ahi1
Fish:	WT
Anatomical Terms:	central nervous system pronephros retinal neural layer whole organism
Stage Range:	1-cell to Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ahi1^lri46/lri46
Observed In:	inner ear otolith post-vent region swim bladder whole organism
Stage:	Day 5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Invest. Ophthalmol. Vis. Sci.