FIGURE

Fig. 5

ID

ZDB-FIG-170227-5

Publication

Lessieur et al., 2017 - The Ciliopathy Gene ahi1 Is Required for Zebrafish Cone Photoreceptor Outer Segment Morphogenesis and Survival

Other Figures

All Figure Page

Back to All Figure Page

Fig. 5

Ultrastructural analysis of wild-type and ahi1^lri46 photoreceptors. (A, B) Transmission electron microscopy provided ultrastructural views of wild-type and ahi1^lri46 photoreceptors. Mutant outer segments are short, fragmented, and disorganized. (C) Higher magnification of wild-type photoreceptor showing tightly stacked disc membranes and inner segment. (D) Vesiculation at the proximal end of an outer segment in ahi1^lri46 mutant (arrowhead). (E) Improper orientation of disc membranes. In this field, an axoneme (white arrow) and disc membranes from outer segments of three different cells, labeled 1 to 3, are oriented perpendicular to each other. (F) Several layers of discs are abnormally wrapped around the outer segment (white arrow). (G, H) Cross-sections through the ciliary pocket (cp) show the proper 9 + 0 microtubule arrangement of axoneme. Mitochondria (m) are visible in (G). Y-shaped crosslinkers also are present (arrows in [G, H]). Staining artifacts are visible as diffuse, black deposits in some panels. Scale bars: 1 μm (A–F) and 200 nm (G, H).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ahi1^lri46/lri46
Observed In:	retinal cone cell axoneme retinal cone cell photoreceptor cell outer segment organization retinal cone cell photoreceptor disc membrane retinal cone cell photoreceptor outer segment
Stage:	Day 5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Invest. Ophthalmol. Vis. Sci.