ZFIN ID: ZDB-FIG-161212-8
Randlett et al., 2013 - Cellular requirements for building a retinal neuropil. Cell Reports   3:282-90 Full text @ Cell Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Condition:
Knockdown Reagents:
Anatomical Terms:
Stage Range: Protruding-mouth to Day 4
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Protruding-mouth

Fig. 5

BC Axons Can Form a Sublaminated Neuropil in the Absence of Partner Neurons and Glia

(A–C) A neuropil layer (arrowhead) forms at the basal surface of a cellularly simplified retina ath5−/−;ptf1a−/−;ptf1aMOs treated with DAPT, lacking ACs, HCs, RGCs, and MG (A). The neuropil layer is actin rich (B) and contains RibeyeA positive staining at 72 hpf (C).

(D) Gross sublaminar structure is maintained in cellularly simplified retinas, with Q19 apical to the PKC staining in the IPL-like layer. This sublamination was statistically significant (p = 6.22 × 10−44, n = 3 sections). Scale bars, 10 μm (A, D, and E) and 20 μm (B, C, F, G, H, and I).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
Cerulean q19Tg + MO1-atoh7 + MO1-ptf1a + MO4-ptf1a + MO4-tp53 chemical treatment: DAPT Day 4 bipolar neuron IHC
Day 4 retinal inner plexiform layer IHC
GFP nns5Tg control Protruding-mouth bipolar neuron IHC
Protruding-mouth retinal inner plexiform layer IHC
ptf1asa126/sa126; nns5Tg + MO1-ptf1a + MO4-ptf1a + MO4-tp53 chemical treatment: DAPT Protruding-mouth bipolar neuron IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Qualifier Anatomy
Ab1-ctbp2a IHC ptf1asa126/sa126; nns5Tg + MO1-ptf1a + MO4-ptf1a + MO4-tp53 chemical treatment: DAPT Protruding-mouth retinal inner plexiform layer ribbon synapse
Ab1-elavl IHC nns5Tg control Protruding-mouth amacrine cell
IHC Protruding-mouth retinal ganglion cell
IHC ptf1asa126/sa126; nns5Tg + MO1-ptf1a + MO4-ptf1a + MO4-tp53 control Protruding-mouth Not Detected retina
Ab2-prkcb IHC q19Tg + MO1-atoh7 + MO1-ptf1a + MO4-ptf1a + MO4-tp53 chemical treatment: DAPT Day 4 bipolar neuron
IHC Day 4 retinal inner plexiform layer
Phenotype Details
Fish Conditions Stage Phenotype
ptf1asa126/sa126; nns5Tg + MO1-ptf1a + MO4-ptf1a + MO4-tp53 control Protruding-mouth amacrine cell absent, abnormal
Protruding-mouth Muller cell absent, abnormal
Protruding-mouth retina ab1-elavl labeling absent, abnormal
Protruding-mouth retinal ganglion cell absent, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Cell Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Cell Rep.