FIGURE

Fig. 5

ID

ZDB-FIG-161212-8

Publication

Randlett et al., 2013 - Cellular requirements for building a retinal neuropil

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Fig. 5

BC Axons Can Form a Sublaminated Neuropil in the Absence of Partner Neurons and Glia

(A–C) A neuropil layer (arrowhead) forms at the basal surface of a cellularly simplified retina ath5^−/−;ptf1a^−/−;ptf1aMOs treated with DAPT, lacking ACs, HCs, RGCs, and MG (A). The neuropil layer is actin rich (B) and contains RibeyeA positive staining at 72 hpf (C).

(D) Gross sublaminar structure is maintained in cellularly simplified retinas, with Q19 apical to the PKC staining in the IPL-like layer. This sublamination was statistically significant (p = 6.22 × 10⁻⁴⁴, n = 3 sections). Scale bars, 10 μm (A, D, and E) and 20 μm (B, C, F, G, H, and I).

Expression Data

Genes:	Cerulean GFP
Antibodies:	Ab1-ctbp2a Ab1-elavl Ab2-prkcb
Fish:	nns5Tg ptf1a^sa126/sa126; nns5Tg + MO1-ptf1a + MO4-ptf1a + MO4-tp53 q19Tg + MO1-atoh7 + MO1-ptf1a + MO4-ptf1a + MO4-tp53
Condition:	chemical treatment: DAPT
Knockdown Reagents:	MO1-atoh7 MO1-ptf1a MO4-ptf1a MO4-tp53
Anatomical Terms:	amacrine cell bipolar neuron retinal ganglion cell retinal inner plexiform layer retinal inner plexiform layer ribbon synapse
Stage Range:	Protruding-mouth to Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ptf1a^sa126/sa126; nns5Tg + MO1-ptf1a + MO4-ptf1a + MO4-tp53
Knockdown Reagents:	MO1-ptf1a MO4-ptf1a MO4-tp53
Observed In:	amacrine cell Muller cell retina retinal ganglion cell
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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