PUBLICATION

Cellular requirements for building a retinal neuropil

Authors
Randlett, O., MacDonald, R.B., Yoshimatsu, T., Almeida, A.D., Suzuki, S.C., Wong, R.O., Harris, W.A.
ID
ZDB-PUB-161116-6
Date
2013
Source
Cell Reports   3: 282-90 (Journal)
Registered Authors
Harris, William A., MacDonald, Ryan, Randlett, Owen, Wong, Rachel
Keywords
none
MeSH Terms
  • Amacrine Cells/cytology
  • Amacrine Cells/pathology
  • Amacrine Cells/ultrastructure
  • Animals
  • Animals, Genetically Modified
  • Embryo, Nonmammalian/metabolism
  • Microscopy, Fluorescence
  • Microscopy, Video
  • Neurons/pathology
  • Neuropil/cytology*
  • Neuropil/pathology
  • Presynaptic Terminals/pathology
  • Retina/pathology
  • Retina/ultrastructure*
  • Retinal Bipolar Cells/cytology
  • Retinal Bipolar Cells/pathology
  • Retinal Bipolar Cells/ultrastructure
  • Retinal Ganglion Cells/cytology
  • Retinal Ganglion Cells/pathology
  • Retinal Ganglion Cells/ultrastructure
  • Synapses/pathology
  • Synapses/ultrastructure
  • Zebrafish/growth & development
PubMed
23416047 Full text @ Cell Rep.
Abstract
How synaptic neuropil is formed within the CNS is poorly understood. The retinal inner plexiform layer (IPL) is positioned between the cell bodies of amacrine cells (ACs) and retinal ganglion cells (RGCs). It consists of bipolar cell (BC) axon terminals that synapse on the dendrites of ACs and RGCs intermingled with projections from Müller glia (MG). We examined whether any of these cellular processes are specifically required for the formation of the IPL. Using genetic and pharmacological strategies, we eliminated RGCs, ACs, and MG individually or in combination. Even in the absence of all of these partner cells, an IPL-like neuropil consisting of only BC axon terminals still forms, complete with presynaptic specializations and sublaminar organization. Previous studies have shown that an IPL can form in the complete absence of BCs; therefore, we conclude that neither presynaptic nor postsynaptic processes are individually essential for the formation of this synaptic neuropil.
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Sequence Targeting Reagents
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Errata and Notes