FIGURE

Fig. S2

ID

ZDB-FIG-161212-3

Publication

Randlett et al., 2013 - Cellular requirements for building a retinal neuropil

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Fig. S2

Elimination of ACs through Ptf1a Disruption, Related to Figure 2

(A) The ptf1a^sa126 mutation is an A to T conversion, which results in a non-sense mutation at amino acid 126 of 265. This results in a truncation within the loop of the basic helix-loop-helix DNA binding domain.

(B and C) Immunostaining of retinal cryosections using (B) 5E11 to stain AC neurites and (C) anti-HuC/D to stain RGC and AC cell bodies, indicates that some ACs remain in homozygous ptf1a^−/− mutants. Injection of ptf1a morpholinos into ptf1a^−/− mutants, causes a further reduction in ACs numbers, where large stretches of retina lack 5E11 staining and HuC/D positive cells in the AC layer (higher magnification inserts given in: i, and ii).

(D) WT retina with the vsx1GFP labeling BC axons in the IPL (arrow) and ACs/RGCs labeled with HuC/D.

(E) In the ath5^−/−;ptf1a^−/−; ptf1aMO, AC/RGC-free retina, the IPL is positioned along the basal surface of the retina (arrow). Few remaining ACs shown with arrowheads.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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