FIGURE

Fig. S1

ID

ZDB-FIG-161201-8

Publication

Shin et al., 2016 - Vegfa signals through ERK to promote angiogenesis, but not artery differentiation

Other Figures

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Fig. S1

Activation of presumptive Vegfa downstream signaling effectors in zebrafish blood vessels. (A-E) Confocal images of wild type Tg(fli1a:egfp)^y1 embryos at 24 hours post fertilization (hpf). Lateral views, anterior to the left, dorsal is up. Dorsal aorta (DA) and posterior cardinal vein (PCV) are indicated. Arrowheads indicate intersomitic vessels, arrows indicate DA endothelial cells, and yellow arrowheads denote PCV endothelial cells. (A'-E') Images from same embryos as in A-E, immuonstained with primary antibody against indicated epitope and secondary antibody conjugated to HRP followed by TSA-Cy3 amplification. (A''- E'') Overlay of images from Tg(fli1a:egfp)^y1 expression and Cy3 fluorescence.

Expression Data

Gene:	GFP
Antibodies:	Ab1-plcg1 Ab2-mapk14 Ab2-plcg1
Fish:	y1Tg
Anatomical Terms:	dorsal aorta endothelial cell intersegmental vessel posterior cardinal vein
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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