FIGURE

Fig. S5

ID

ZDB-FIG-161201-12

Publication

Shin et al., 2016 - Vegfa signals through ERK to promote angiogenesis, but not artery differentiation

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Fig. S5

ERK inhibition normalized the hyper-angiogenic phenotype in Notch deficient embryos. (A) Proportion of embryos with expression level of indicated marker as determined by in situ hybridization. For flt4, “ectopic” denotes expression in the dorsal aorta, where it is normally absent. See panel Figure 7F-K for corresponding images. (B) Tg(kdrl:egfp)^la116 embryos injected with 15 ng control (5misMO) or 15 ng dll4 MO. Embryos were treated from 48 hpf to 72 hpf with indicated compound. (C) Quantification of ectopic ISV branches in 5misMO- or dll4 MO-injected embryos treated with DMSO or 15 μM SL327 as in (A). Numbers of cells and embryos used for analysis are shown. Error bars are ±SD. ***p<0.001.

Expression Data

Gene:	EGFP
Fish:	la116Tg la116Tg + MO2-dll4
Condition:	chemical treatment by environment: SL-327
Knockdown Reagent:	MO2-dll4
Anatomical Term:	intersegmental vessel
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	la116Tg + MO2-dll4
Condition:	chemical treatment by environment: SL-327
Knockdown Reagent:	MO2-dll4
Observed In:	intersegmental vessel
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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