FIGURE

Fig. 2

ID

ZDB-FIG-161201-2

Publication

Shin et al., 2016 - Vegfa signals through ERK to promote angiogenesis, but not artery differentiation

Other Figures

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Fig. 2

ERK phosphorylation in sprouting ISVs requires Vegfa signaling. (A-G) Confocal images of Tg(fli1a:egfp)^y1 embryos at 21 hpf (A-C′) and 20 hpf (D-G) immunostained for GFP (green) and pERK (red). Lateral views, anterior to the left, dorsal is up. Number of analyzed embryos is indicated, representative images are shown. Embryos were injected with 100 pg mcherry mRNA (A,A′), 25 pg vegf₁₂₁ mRNA (B,B′) or 100 pg vegfc mRNA (C,C′). (D) Wild-type embryo. (E) kdrl^y17 mutant embryo. (F) flt4^um131 mutant embryo. (G) plcg1^y13 mutant embryo. Arrows indicate pERK-positive cells, or lack thereof. (H) Graph showing number of pERK⁺ endothelial cells per embryo at 20 hpf in a defined segment of the DA in kdrl and flt4 mutant embryos, or in embryos treated with 10 µM SU5416. Number of embryos for each genotype or treated with compound is indicated on the x-axis. Error bars represent s.d.; ***P<0.001; ns, not statistically significant.

Expression Data

Gene:	GFP
Antibody:	Ab9-mapk
Fish:	y1Tg kdrl^y17/y17; y1Tg plcg1^y13/y13; y1Tg kdrl^y17; flt4^um131/um131
Anatomical Term:	endothelial cell
Stage:	20-25 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	kdrl^y17/y17; y1Tg plcg1^y13/y13; y1Tg kdrl^y17; flt4^um131/um131
Observed In:	endothelial cell
Stage:	20-25 somites

Phenotype Detail

Acknowledgments

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