ZFIN ID: ZDB-FIG-161107-8
Marín-Juez et al., 2016 - Fast revascularization of the injured area is essential to support zebrafish heart regeneration. Proceedings of the National Academy of Sciences of the United States of America   113(40):11237-11242 Full text @ Proc. Natl. Acad. Sci. USA
ADDITIONAL FIGURES
EXPRESSION / LABELING:
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Anatomical Terms:
Stage: Adult
PHENOTYPE:
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Observed In:
Stage: Adult

Fig. 2

vegfaa mutants exhibit delayed and disorganized revascularization. (A) qPCR analysis of vegfaa expression at 6 hpi and 1, 2, 3, and 5 dpi. Data are expressed as mean ± SD, *P < 0.05 (n = 4). (B and C) Tg(etv2:EGFP) vegfaa-/- ventricles at 40 hpi (n = 3). White arrowheads point to coronary vessels sprouting into the injured area. (D and E) Tg(etv2:EGFP) WT (n = 5) and Tg(etv2:EGFP) vegfaa-/- ventricles at 4 dpi (n = 3). Asterisk marks an area of coronary plexus. Dotted lines delineate the injured area. (F and G) Sections of WT (n = 4) and vegfaa-/- (n = 4) ventricles at 45 dpi stained with AFOG to identify scar (blue), fibrin (red), and muscle (orange). (H) Microarray profiling of vegfaa+/+ and vegfaa-/- ventricles. Data are shown relative to vegfaa+/+ ventricles, which are set at 1 (dashed line). (Scale bars: BE, 50 µm; F and G, 100 µm.)

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
ccbe1 vegfaabns1/bns1 standard conditions Adult cardiac ventricle RTPCR
EGFP ci1Tg cold damage: heart Adult heart vasculature regenerating tissue IHC
Adult regenerating tissue vascular sprouts IHC
vegfaabns1/bns1; ci1Tg cold damage: heart Adult heart vasculature regenerating tissue IHC
Adult regenerating tissue vascular sprouts IHC
plg vegfaabns1/bns1 standard conditions Adult cardiac ventricle RTPCR
vegfaa WT cold damage: heart Adult heart RTPCR
vegfab vegfaabns1/bns1 standard conditions Adult cardiac ventricle RTPCR
Adult heart vasculature regenerating tissue RTPCR
Adult regenerating tissue vascular sprouts RTPCR
vegfc vegfaabns1/bns1 standard conditions Adult cardiac ventricle RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT cold damage: heart Adult heart vegfaa expression increased amount, abnormal
vegfaabns1/bns1 standard conditions Adult cardiac ventricle ccbe1 expression increased amount, abnormal
Adult cardiac ventricle plg expression increased amount, abnormal
Adult cardiac ventricle vegfab expression increased amount, abnormal
Adult cardiac ventricle vegfc expression increased amount, abnormal
Adult heart regeneration decreased process quality, abnormal
vegfaabns1/bns1; ci1Tg cold damage: heart Adult regenerating tissue coronary vasculature development delayed, abnormal
Adult regenerating tissue heart vasculature disorganized, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA