FIGURE

Fig. 2

ID

ZDB-FIG-161107-8

Publication

Marín-Juez et al., 2016 - Fast revascularization of the injured area is essential to support zebrafish heart regeneration

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Fig. 2

vegfaa mutants exhibit delayed and disorganized revascularization. (A) qPCR analysis of vegfaa expression at 6 hpi and 1, 2, 3, and 5 dpi. Data are expressed as mean ± SD, *P < 0.05 (n = 4). (B and C) Tg(etv2:EGFP) vegfaa^-/- ventricles at 40 hpi (n = 3). White arrowheads point to coronary vessels sprouting into the injured area. (D and E) Tg(etv2:EGFP) WT (n = 5) and Tg(etv2:EGFP) vegfaa^-/- ventricles at 4 dpi (n = 3). Asterisk marks an area of coronary plexus. Dotted lines delineate the injured area. (F and G) Sections of WT (n = 4) and vegfaa^-/- (n = 4) ventricles at 45 dpi stained with AFOG to identify scar (blue), fibrin (red), and muscle (orange). (H) Microarray profiling of vegfaa^+/+ and vegfaa^-/- ventricles. Data are shown relative to vegfaa^+/+ ventricles, which are set at 1 (dashed line). (Scale bars: B–E, 50 µm; F and G, 100 µm.)

Expression Data

Genes:	ccbe1 EGFP plg vegfaa vegfab vegfc
Fish:	WT vegfaa^bns1/bns1 ci1Tg vegfaa^bns1/bns1; ci1Tg
Condition:	cold damage: heart
Anatomical Terms:	cardiac ventricle heart heart vasculature regenerating tissue regenerating tissue vascular sprouts
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT vegfaa^bns1/bns1 vegfaa^bns1/bns1; ci1Tg
Condition:	cold damage: heart
Observed In:	cardiac ventricle heart heart regeneration regenerating tissue coronary vasculature development regenerating tissue heart vasculature
Stage:	Adult

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA