FIGURE

Fig. 4

ID
ZDB-FIG-161107-15
Publication
Marín-Juez et al., 2016 - Fast revascularization of the injured area is essential to support zebrafish heart regeneration
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Fig. 4

Revascularization of the damaged area supports CM proliferation. (A and C) Images of the apex of Tg(etv2:EGFP) WT and Tg(hsp70l:dn-vegfaa);Tg(etv2:EGFP) ventricles at 7 dpi. WT and Tg(hsp70l:dn-vegfaa) were daily heatshocked. (B and D) Sections of Tg(etv2:EGFP) WT and Tg(hsp70l:dn-vegfaa);Tg(etv2:EGFP) ventricles. CMs are immunostained with anti-MHC antibody (red), and nuclei counterstained with DAPI (blue). Insets show high-magnification images of the coronaries on the surface of the heart (yellow arrows) and in the damaged area (white arrows). (E) Quantification of coronary vessel formation in the injured area. The percentage of etv2:EGFP+ area was measured relative to total injured area in WT ventricles (n = 6) and Tg(hsp70l:dn-vegfaa) ventricles (n = 5). (F and G) Sections from Tg(etv2:EGFP) WT and Tg(hsp70l:dn-vegfaa);Tg(etv2:EGFP) ventricles stained with anti-Mef2 (red) and anti-PCNA (green) antibodies. Arrowheads point to Mef2+/PCNA+ nuclei. Insets show high-magnification images of Mef2+/PCNA+ nuclei. (H) Quantification of CM proliferation in WT (n = 5) and Tg(hsp70l:dn-vegfaa) (n = 5) ventricles at 7 dpi. Data are expressed as mean ± SEM, **P < 0.001. Dotted lines delineate the injured area. (Scale bars: 100 µm.)

Expression Data
Gene:
Antibody:
Fish:
Conditions:
Anatomical Terms:
Stage: Adult

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Condition:
Observed In:
Stage: Adult

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA