FIGURE

Fig. 2

ID

ZDB-FIG-161018-21

Publication

Olsen et al., 2016 - G9a and ZNF644 Physically Associate to Suppress Progenitor Gene Expression during Neurogenesis

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Fig. 2

Zebrafish znf644 Paralogs Are Specifically Expressed In and Regulate the Development of Retinal and Midbrain Progenitor Cells

(A) Conservation of C2H2-like ZF motifs between human and zebrafish ZNF644 genes.

(B) Representative lateral views, retinal cross-sections, and midbrain cross-sections of whole-mount in situ hybridization (WISH) assays monitoring znf644a or znf644b expression at 24 hpf (n > 15 in each group). Arrows denote the inner part of the central retinal epithelium.

(C) Lateral views of g9a (20/32), znf644a (10/18), or znf644b (14/21) morphant embryos compared with WT (21/21). Dashed circles denote 2D area of the WT retina. Arrowheads denote the dorsal hindbrain region.

(D) Quantitation (mean ± SD) of retinal 2D cross-sectional area (¼m²) of znf644a, znf644b, or g9a morphants relative to WT embryos. Error bars represent SD. *p < 0.005, Student′s t test.

(E) RT-PCR assays monitoring unspliced (unspl) and spliced (spl) znf644a or znf644b transcripts at 24 hpf.

Expression Data

Genes:	znf644a znf644b
Fish:	AB
Anatomical Terms:	midbrain presumptive retinal pigmented epithelium
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO1-ehmt2 AB + MO1-znf644a AB + MO1-znf644b
Knockdown Reagents:	MO1-ehmt2 MO1-znf644a MO1-znf644b
Observed In:	eye midbrain
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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