|ZFIN ID: ZDB-FIG-160726-4|
Endocardial Intracellular Calcium Level Correlates with the Intracardiac FH Index and Depends on TRPs Channels Function
(A and B) Calcium level quantification in the embryonic heart of controls (n = 10), mutants of heart contraction (cmlc1 knockdown; n = 3), lidocaine-treated embryos (n = 3), gata1 (n = 3), gata2 (n = 6) MO, and gata2 MO plus 4α-PDD (n = 2). At 48 hpf, fishes with a reduced oscillatory flow index (sih, lidocaine, cmlc1, and gata2) show a significant reduction of intracellular calcium intensity in the AVC (white arrow), whereas the specific ligand of TRPV4 (4α-PDD) channels restores an almost normal intracellular calcium intensity in the AVC of the gata2 MO. *p < 0.05; **p < 0.01 ANOVA. Error bars indicate the SD.
(C) Calcium level quantification in the embryonic heart of control, trpp2 MO (n = 3), trpp2cup-/- (n = 6), trpv4 MO (n = 6), and trpv4sa1671-/- (n = 2) embryos. Error bars indicate the SD.
(D) 4α-PDD induces an increase in intracellular calcium in the absence of flow in the Tg(fli1a:gcamp3.0), sih (n = 5) hearts. ***p < 0.001 t test. Error bars indicate the SD.
(E) Confocal micrograph showing the effect of 4α-PDD on calcium signaling in sih.
(F) Confocal micrograph and intensity quantification of klf2a:H2B-eGFP intensity in gata2 knockdown with and without 4α-PDD treatment (n = 9 and n = 3, respectively). Error bars indicate the SD.
(G) Schematic highlighting a potential mechanism for oscillatory flow mechanodetection by the TRP channels (in red) specifically in the AVC.
|Acknowledgments:||This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Curr. Biol.|