FIGURE

Fig. 8

ID

ZDB-FIG-160315-23

Publication

Govindan et al., 2016 - Cx43-Dependent Skeletal Phenotypes Are Mediated by Interactions between the Hapln1a-ECM and Sema3d during Fin Regeneration

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Fig. 8

Morpholino mediated knockdown of hapln1a results in reduced Sema3d protein levels.

(A) Longintudinal section of fin rays treated with control morpholino and sema3d targeting morpholino (Sema3d KD). Immuno-staining for Sema3d (red) and counterstained for nuclei with TOPRO (blue). Compared to the control MM treated fins, Sema3d MO treated fins show reduced staining for Sema3d. (B) Longitudinal section of fin rays treated with hapln1a control morpholino (Hapln1a MM) and hapln1a targeting morpholino (Hapln1a MO). Immuno-staining for Sema3d (blue) and counterstained for nuclei with Propidium Iodide (PI, red). Compared to the control MM treated fins, Hapln1a MO treated fins show reduced staining for Sema3d. Arrow identifies the basal layer of epithelium; arrow head identifies the bone; m, mesenchyme; e, epithelium. Scale bar represents 20 µm.

Expression Data

Antibody:	Ab1-sema3d
Fish:	AB/C32 AB/C32 + MO2-hapln1a AB/C32 + MO5-sema3d
Condition:	amputation: caudal fin
Knockdown Reagents:	MO2-hapln1a MO5-sema3d
Anatomical Term:	regenerating fin
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB/C32 + MO2-hapln1a AB/C32 + MO5-sema3d
Condition:	amputation: caudal fin
Knockdown Reagents:	MO2-hapln1a MO5-sema3d
Observed In:	regenerating fin
Stage:	Adult

Phenotype Detail

Acknowledgments

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