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ZFIN ID: ZDB-FIG-160315-18
Govindan et al., 2016 - Cx43-Dependent Skeletal Phenotypes Are Mediated by Interactions between the Hapln1a-ECM and Sema3d during Fin Regeneration. PLoS One   11:e0148202 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage: Adult

Fig. 1

In situ hybridization showing the expression of Hapln1a-ECM components on 5 dpa regenerating fins.

(A) Whole mount in situ hybridization of components of the Hapln1a-ECM. The genes has1, has2, and acanb are expressed during fin regeneration. The amputation plane is indicated by a black line in all the panels. Scale bar represents 100µm. (B) In situ hybridization on a WT 5 dpa cryo-section reveals compartmental expression of has1, has2, and acanb. Blastema (b), mesenchyme (m), and skeletal precursor cells (*). The thick arrow identifies the basal layer of the epidermis, which underlies the epidermis (e). The thin arrow identifies lepidotrichia and the arrowhead identifies the actinotrichia. Scale bar represents 50µm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
acanb AB/C32 amputation: caudal fin Adult blastema ISH
Adult regenerating fin ISH
Adult regenerating fin mesenchyme ISH
has1 AB/C32 amputation: caudal fin Adult basal regeneration epithelium ISH
Adult blastema ISH
Adult regenerating fin ISH
Adult regenerating fin mesenchyme ISH
has2 AB/C32 amputation: caudal fin Adult blastema ISH
Adult regenerating fin ISH
Adult regenerating fin mesenchyme ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One