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ZIRC
ZFIN ID: ZDB-FIG-160315-21
Govindan et al., 2016 - Cx43-Dependent Skeletal Phenotypes Are Mediated by Interactions between the Hapln1a-ECM and Sema3d during Fin Regeneration. PLoS One   11:e0148202 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Condition:
Knockdown Reagent:
Anatomical Term:
Stage: Adult
PHENOTYPE:
Fish:
Condition:
Knockdown Reagent:
Observed In:
Stage: Adult

Fig. 5

Morpholino mediated knockdown of hapln1a results in reduced Acan protein levels.

Longitudinal section of fin rays treated with hapln1a control morpholino (Hapln1a MM) and hapln1a targeting morpholino (Hapln1a MO). Immuno-staining for Acan (blue) and counterstained for nuclei with Propidium Iodide (PI, red). Compared to the control Hapln1a MM treated fins, Hapln1a MO treated fins show reduced staining for Acan. Arrow identifies the basal layer of epithelium; arrow head identifies the bone; m, mesenchyme; e, epithelium. Arrow identifies the basal layer of epithelium; arrow head identifies the bone; m, mesenchyme; e, epithelium. Scale bar represents 20 ┬Ám.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
acanb Ab1-acan AB/C32 amputation: caudal fin Adult regenerating fin IHC
Ab1-acan AB/C32 + MO2-hapln1a amputation: caudal fin Adult regenerating fin IHC
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB/C32 + MO2-hapln1a amputation: caudal fin Adult regenerating fin acanb expression decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One