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ZIRC
ZFIN ID: ZDB-FIG-160315-22
Govindan et al., 2016 - Cx43-Dependent Skeletal Phenotypes Are Mediated by Interactions between the Hapln1a-ECM and Sema3d during Fin Regeneration. PLoS One   11:e0148202 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Condition:
Knockdown Reagents:
Anatomical Term:
Stage: Adult
PHENOTYPE:
Fish:
Condition:
Knockdown Reagents:
Observed In:
Stage: Adult

Fig. 6

Reduced Acan contributes to Hapln1a knockdown phenotypes.

(A) Longitudinal section of fin rays treated with standard control morpholino (Standard control MO) and acanb ATG-blocking morpholino (Acanb MO1) or splice blocking morpholino (Acanb MO2). Immuno-staining for Acan (red) and counterstained for nuclei with To-pro (blue). Compared to the standard MO treated fins, Acanb MO treated fins show reduced staining for Acan. White arrow identifies the basal layer of epithelium; yellow arrow identifies Acan staining in the lepidotrichia, arrow head identifies the bone; m, mesenchyme; e, epithelium. Scale bar represents 20 ┬Ám. (B) Bar graph shows that regenerate length, segment length and cell proliferation are significantly reduced upon acanb knockdown using both MO1 and MO2. The mean of percent similarity for the MO treated experimental group and the corresponding control group were estimated and compared. Statistical significance was determined using the student′s t-test (P<0.05) and the error bars represent standard error of mean. The hashed bars indicate the extent of hapln1a knockdown effect compared to acanb knockdown. (C) Representative alizarin red stained fins showing extent of bone calcification. The black line indicates the amputation plane. (D) The extent of mineralization was calculated as the ratio of the zone of mineralization (extent of detectable alizarin red staining length) to the total regenerate length. The mean of percent similarity for the MO treated experimental group and the corresponding control group were estimated and compared, and the statistical significance between the groups was determined using two tailed unpaired student′s t-test (P<0.05) and the error bars indicate the standard error of mean.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
acanb Ab1-acan AB/C32 amputation: caudal fin Adult regenerating fin IHC
Ab1-acan AB/C32 + MO1-acanb amputation: caudal fin Adult regenerating fin IHC
Ab1-acan AB/C32 + MO2-acanb amputation: caudal fin Adult regenerating fin IHC
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB/C32 + MO1-acanb amputation: caudal fin Adult regenerating fin acanb expression decreased amount, abnormal
Adult regenerating fin decreased length, abnormal
Adult regenerating fin bone mineralization decreased occurrence, abnormal
Adult regenerating fin cell population proliferation decreased occurrence, abnormal
Adult regenerating fin lepidotrichium segment decreased length, abnormal
AB/C32 + MO2-acanb amputation: caudal fin Adult regenerating fin acanb expression decreased amount, abnormal
Adult regenerating fin decreased length, abnormal
Adult regenerating fin bone mineralization decreased occurrence, abnormal
Adult regenerating fin cell population proliferation decreased occurrence, abnormal
Adult regenerating fin lepidotrichium segment decreased length, abnormal
AB/C32 + MO2-hapln1a amputation: caudal fin Adult regenerating fin decreased length, abnormal
Adult regenerating fin cell population proliferation decreased occurrence, abnormal
Adult regenerating fin lepidotrichium segment decreased length, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One