FIGURE

Fig. 3

ID

ZDB-FIG-160208-18

Publication

Sisson et al., 2015 - A role of glypican4 and wnt5b in chondrocyte stacking underlying craniofacial cartilage morphogenesis

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Fig. 3

Cranial neural crest cell migration and specification is unaffected in gpc4 and wnt5b mutants. (A–O) Neural crest specification appears normal within gpc4 and wnt5b mutants Scale bar = 100 µm. (A–I) Pre-migratory neural crest markers. 6 somites. (J–O)Pharyngeal arch markers. (J–L) 28 hpf. (M–O) 32 hpf. (P–R) Migration of neural crest appears to be unaffected in gpc4 and wnt5b mutants when compared to wild type fish. Tg(fli1a:EGFP)^y1 embryos have neural crest derived cells labeled by GFP expression. Embryos were fixed at 33 hpf and stained with the Alcama (Zn5) antibody (red). Pharyngeal arches are numbered. Scale bar = 50 µm.

Expression Data

Genes:	dlx2a foxd3 hand2 pax3a sox10
Fish:	WT gpc4^fr6/fr6 wnt5b^ta98/ta98
Anatomical Terms:	pharyngeal arch premigratory neural crest
Stage Range:	5-9 somites to Prim-15

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	gpc4^fr6/fr6 wnt5b^ta98/ta98 gpc4^fr6/fr6; y1Tg wnt5b^ta98/ta98; y1Tg
Observed In:	convergent extension neural crest cell fate specification neural crest cell migration whole organism anterior-posterior axis
Stage Range:	5-9 somites to Prim-15

Phenotype Detail

Acknowledgments

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Reprinted from Mechanisms of Development, 138 Pt 3, Sisson, B.E., Dale, R.M., Mui, S.R., Topczewska, J.M., Topczewski, J., A role of glypican4 and wnt5b in chondrocyte stacking underlying craniofacial cartilage morphogenesis, 279-90, Copyright (2015) with permission from Elsevier. Full text @ Mech. Dev.