ZFIN ID: ZDB-FIG-160205-131
Zhang et al., 2015 - The transcriptional coactivator Taz regulates proximodistal patterning of the pronephric tubule in zebrafish. Mechanisms of Development   138 Pt 3:328-35 Full text @ Mech. Dev.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Conditions:
Knockdown Reagents:
Observed In:
Stage Range: Prim-5 to Long-pec

Fig. 5

Effects of RA and DEAB on patterning of the pronephric tubule in control embryos and taz morphants. Visualization of pronephric tubule segments in 24 and 48 hpf zebrafish embryos by anti-GFP (red) staining of the ET11-9 segment, followed by α6F staining of the pronephric tubule (green). Panels show untreated (DMSO) control zebrafish embryos and taz morphants, as well as embryos treated with RA or DEAB in DMSO. RA treatment shortened the DL segments in both control (D) and 24 hpf taz morphant (H), but extended the ET11-9 segments in control embryo (D) but not in the 24 hpf taz morphant (H). DEAB extended the DL segments in both control (I, J) and 24 hpf taz morphants (M, N) as well as in control (Q, R) and taz morphants at 48 hpf (U, V), and reversed PCT dilation in 48 hpf taz morphants (V compared with T).

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
sqet11Et chemical treatment: 4-(diethylamino)benzaldehyde Prim-5 pronephric distal early tubule decreased length, abnormal
Prim-5 pronephric distal late tubule increased length, abnormal
Prim-5 pronephric proximal convoluted tubule decreased length, abnormal
Prim-5 pronephric proximal straight tubule decreased length, abnormal
Long-pec pronephric distal late tubule increased length, abnormal
sqet11Et chemical treatment by environment: retinoic acid Prim-5 pronephric distal early tubule increased length, abnormal
Prim-5 pronephric distal late tubule decreased length, abnormal
Prim-5 pronephric proximal convoluted tubule dilated, abnormal
Prim-5 pronephric proximal convoluted tubule increased length, abnormal
Prim-5 pronephric proximal straight tubule increased length, abnormal
sqet11Et + MO1-wwtr1 + MO4-tp53 control Prim-5 pronephric distal early tubule decreased length, abnormal
Prim-5 pronephric proximal convoluted tubule increased length, abnormal
Prim-5 pronephric proximal straight tubule decreased length, abnormal
Long-pec pronephric proximal convoluted tubule dilated, abnormal
sqet11Et + MO1-wwtr1 + MO4-tp53 chemical treatment: 4-(diethylamino)benzaldehyde Prim-5 pronephric distal late tubule increased length, abnormal
Prim-5 pronephric proximal convoluted tubule decreased length, abnormal
Long-pec pronephric distal late tubule increased length, abnormal
Long-pec pronephric proximal convoluted tubule size, ameliorated
sqet11Et + MO1-wwtr1 + MO4-tp53 chemical treatment by environment: retinoic acid Prim-5 pronephric distal early tubule decreased length, abnormal
Prim-5 pronephric proximal convoluted tubule dilated, exacerbated
Prim-5 pronephric proximal convoluted tubule increased length, exacerbated
Acknowledgments:
ZFIN wishes to thank the journal Mechanisms of Development for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Mechanisms of Development, 138 Pt 3, Zhang, J., Yuan, S., Vasilyev, A., Amin Arnaout, M., The transcriptional coactivator Taz regulates proximodistal patterning of the pronephric tubule in zebrafish, 328-35, Copyright (2015) with permission from Elsevier. Full text @ Mech. Dev.