The transcriptional coactivator Taz regulates proximodistal patterning of the pronephric tubule in zebrafish

Zhang, J., Yuan, S., Vasilyev, A., Amin Arnaout, M.
Mechanisms of Development   138 Pt 3: 328-35 (Journal)
Registered Authors
Vasilyev, Aleksandr, Yuan, Shipeng, Zhang, Jiaojiao
Taz, kidney cysts, patterning, pronephros, zebrafish
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Body Patterning/drug effects
  • Body Patterning/genetics
  • Body Patterning/physiology
  • Cell Count
  • Gene Expression Regulation, Developmental
  • Gene Knockdown Techniques
  • Intracellular Signaling Peptides and Proteins/antagonists & inhibitors
  • Intracellular Signaling Peptides and Proteins/genetics
  • Intracellular Signaling Peptides and Proteins/physiology*
  • Kidney Tubules/cytology
  • Kidney Tubules/embryology*
  • Kidney Tubules/metabolism
  • Mesoderm/cytology
  • Mesoderm/embryology
  • Mesoderm/metabolism
  • Pronephros/cytology
  • Pronephros/embryology*
  • Pronephros/metabolism
  • Tretinoin/metabolism
  • Tretinoin/pharmacology
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish/physiology*
  • Zebrafish Proteins/antagonists & inhibitors
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/physiology*
26248207 Full text @ Mech. Dev.
The zebrafish pronephric tubule consists of a proximal and distal segments and a collecting duct. The proximal segment is subdivided into the neck, proximal convoluted (PCT) and proximal straight (PST) tubule segments. The distal segment consists of a distal-early (DE) and late (DL) segments. How the proximal and distal segments develop along the anteroposterior axis is poorly understood. Here we show that knockdown of taz in zebrafish caused shortening and a significant reduction in number of principal cells of the PST-DE segment, and proximalization of the pronephric tubule in 24 hpf embryos. RA treatment expanded the pronephric proximal domain in normal embryos as in taz morphants, an effect that was further enhanced upon exposure of taz morphants to RA. The early pronephric defects in 24 hpf taz morphants led to failure of anterior pronephric tubule migration and convolution, and to PCT dilation and cyst formation in older embryos. In situ hybridization showed weak and transient expression of taz at the bud stage in intermediate mesoderm, the source of pronephric progenitors. The present findings show that Taz is required in anteroposterior patterning of the pronephric progenitor domain in intermediate mesoderm, acting in part by regulating RA signaling in pronephric progenitor field in intermediate mesoderm.
Genes / Markers
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Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes