Fig. 3

The ET11-9 segment is shortened in 24 and 48 hpf taz morphants. (A) The relative lengths of the ET11-9 segment in the normal pronephric tubule. The ET11-9 segment was stained first with anti-GFP (red), followed by α6F antibody (green). The α6F antibody stains the pronephric tubule and duct, but the red fluorescence staining of the ET11-9 segments blocked subsequent α6F green fluorescence staining, so the α6F appeared to stain the two tubular domains flanking the ET11-9 segment. The ET11-9 segments were shortened and PCT segments lengthened in 24 hpf taz morphants. The PCT segment also became dilated and the ventrally bent DL segments were slightly shortened in the 48 hpf taz morphant. (B) Reduced cell proliferation in the ET11-9 segments of the taz morphant at 24 hpf. (a, b) Individual optical sections; (c, d) projected images of stacks. Embryos were stained with anti-GFP (red), followed by anti-BrdU (green) staining of proliferating cells. Bars, 20µm.