ZFIN ID: ZDB-FIG-160205-129
Zhang et al., 2015 - The transcriptional coactivator Taz regulates proximodistal patterning of the pronephric tubule in zebrafish. Mechanisms of Development   138 Pt 3:328-35 Full text @ Mech. Dev.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: Prim-5 to Long-pec
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Prim-5 to Long-pec

Fig. 3

The ET11-9 segment is shortened in 24 and 48 hpf taz morphants. (A) The relative lengths of the ET11-9 segment in the normal pronephric tubule. The ET11-9 segment was stained first with anti-GFP (red), followed by α6F antibody (green). The α6F antibody stains the pronephric tubule and duct, but the red fluorescence staining of the ET11-9 segments blocked subsequent α6F green fluorescence staining, so the α6F appeared to stain the two tubular domains flanking the ET11-9 segment. The ET11-9 segments were shortened and PCT segments lengthened in 24 hpf taz morphants. The PCT segment also became dilated and the ventrally bent DL segments were slightly shortened in the 48 hpf taz morphant. (B) Reduced cell proliferation in the ET11-9 segments of the taz morphant at 24 hpf. (a, b) Individual optical sections; (c, d) projected images of stacks. Embryos were stained with anti-GFP (red), followed by anti-BrdU (green) staining of proliferating cells. Bars, 20┬Ám.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP sqet11Et control Prim-5 pronephric distal early tubule IHC
Prim-5 pronephric proximal straight tubule IHC
Long-pec pronephric distal early tubule IHC
Long-pec pronephric proximal straight tubule IHC
sqet11Et + MO1-wwtr1 + MO4-tp53 standard conditions Prim-5 pronephric distal early tubule IHC
Prim-5 pronephric proximal straight tubule IHC
Long-pec pronephric distal early tubule IHC
Long-pec pronephric proximal straight tubule IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-atp1a1 IHC sqet11Et control Prim-5 pronephric duct
IHC Long-pec pronephric duct
IHC sqet11Et + MO1-wwtr1 + MO4-tp53 standard conditions Prim-5 pronephric duct
IHC Long-pec pronephric duct
Phenotype Details
Fish Conditions Stage Phenotype
sqet11Et + MO1-wwtr1 + MO4-tp53 standard conditions Prim-5 pronephric distal early tubule decreased length, abnormal
Prim-5 pronephric duct multi-ciliated epithelial cell amount, normal
Prim-5 pronephric duct single ciliated epithelial cell decreased amount, abnormal
Prim-5 pronephric proximal convoluted tubule increased length, abnormal
Prim-5 pronephric proximal straight tubule decreased length, abnormal
Long-pec pronephric distal early tubule decreased length, abnormal
Long-pec pronephric distal late tubule decreased length, abnormal
Long-pec pronephric proximal convoluted tubule dilated, abnormal
Long-pec pronephric proximal straight tubule decreased length, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Mechanisms of Development for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Mechanisms of Development, 138 Pt 3, Zhang, J., Yuan, S., Vasilyev, A., Amin Arnaout, M., The transcriptional coactivator Taz regulates proximodistal patterning of the pronephric tubule in zebrafish, 328-35, Copyright (2015) with permission from Elsevier. Full text @ Mech. Dev.