ZFIN ID: ZDB-FIG-160205-127
Zhang et al., 2015 - The transcriptional coactivator Taz regulates proximodistal patterning of the pronephric tubule in zebrafish. Mechanisms of Development   138 Pt 3:328-35 Full text @ Mech. Dev.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: Prim-5 to Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Prim-5 to Protruding-mouth

Fig. 1

Anterior migration of PCT and morphogenesis of the neck segment are impaired in taz morphants. (A) In situ hybridization of embryos with the PCT-specific probe, slc20a1a. Note the absence of anterior migration and convolution of PCT in taz morphants. (B) Neck region morphogenesis is arrested in taz morphants. In situ hybridization using pax2a probe (red), followed by anti-BrdU staining (green) to visualize the neck region morphologies and detect proliferating cells in the neck regions in control embryos (a, c, e, g, i) and taz morphants (b, d, f, h, j). Note in f, the neck regions remained aligned with the A–P axis in the taz morphant, rather than repositioned to align with the mediolateral axis as in the normal embryo (e). (g, h) Individual optical sections of stacks. (i, j) Projected images of stacks. Anterior is to the top. Bars, 20µm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
pax2a WT control Prim-5 pronephric tubule ISH
Prim-25 pronephric tubule ISH
Long-pec pronephric tubule ISH
WT + MO1-wwtr1 + MO4-tp53 standard conditions Prim-5 pronephric tubule ISH
Prim-25 pronephric tubule ISH
Long-pec pronephric tubule ISH
slc20a1a WT control Prim-5 pronephric proximal convoluted tubule ISH
Long-pec pronephric proximal convoluted tubule ISH
Protruding-mouth pronephric proximal convoluted tubule ISH
WT + MO1-wwtr1 + MO4-tp53 standard conditions Prim-5 pronephric proximal convoluted tubule ISH
Long-pec pronephric proximal convoluted tubule ISH
Protruding-mouth pronephric proximal convoluted tubule ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-wwtr1 + MO4-tp53 standard conditions Prim-5 pronephric proximal convoluted tubule position, normal
Long-pec pronephric proximal convoluted tubule mislocalised posteriorly, abnormal
Long-pec pronephric proximal tubule development process quality, abnormal
Long-pec pronephric tubule orientation whole organism anterior-posterior axis, abnormal
Protruding-mouth pronephric proximal convoluted tubule straight, abnormal
Protruding-mouth pronephric proximal tubule development process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Mechanisms of Development for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Mechanisms of Development, 138 Pt 3, Zhang, J., Yuan, S., Vasilyev, A., Amin Arnaout, M., The transcriptional coactivator Taz regulates proximodistal patterning of the pronephric tubule in zebrafish, 328-35, Copyright (2015) with permission from Elsevier. Full text @ Mech. Dev.